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Literature summary extracted from

  • Ferreiro, C.; Heredia, C.F.
    Purification and characterization of an aminoacyl-tRNA hydrolase from the filamentous fungus Fusarium culmorum (1986), Biochim. Biophys. Acta, 882, 410-418.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.29 deacetylated tRNA
-
 Fusarium culmorum
3.1.1.29 EDTA 5 mM, complete inhibition Fusarium culmorum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.29 0.0008
-
 N-acetyl-Phe-tRNA pH 7.5, 30°C Fusarium culmorum
3.1.1.29 0.0008
-
 Phe-tRNA pH 7.5, 30°C Fusarium culmorum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.29 Ca2+ activation is 25% lower than with Mg2+ or Mn2+ Fusarium culmorum
3.1.1.29 Mg2+ maximal activity is obtained with 20 mM Mg2+ or Mn2+ Fusarium culmorum
3.1.1.29 Mn2+ maximal activity is obtained with 20 mM Mg2+ or Mn2+ Fusarium culmorum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.29 46000
-
 gel filtration Fusarium culmorum

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.29 Fusarium culmorum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.29 partial Fusarium culmorum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.29 0.0051
-
-
 Fusarium culmorum

Storage Stability

EC Number Storage Stability Organism
3.1.1.29 -10°C, in presence of 1 mg/ml bovine serum albumin, no loss of activity Fusarium culmorum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.29 Ala-tRNA + H2O
-
 Fusarium culmorum Ala + tRNA
-
 ?
3.1.1.29 Leu-tRNA + H2O
-
 Fusarium culmorum Leu + tRNA
-
 ?
3.1.1.29 Lys-tRNA + H2O
-
 Fusarium culmorum Lys + tRNA
-
 ?
3.1.1.29 Met-tRNA + H2O
-
 Fusarium culmorum Met + tRNA
-
 ?
3.1.1.29 additional information enzyme shows species specificity. Aminoacyl-tRNAs in which the tRNA comes from eucaryotes are equally efficient as substrates of the enzyme, when tRNA comes from procaryotic organisms it is hydrolyzed 40% less efficiently Fusarium culmorum ?
-
 ?
3.1.1.29 N-acetyl-Ala-tRNA + H2O
-
 Fusarium culmorum N-acetyl-Ala + tRNA
-
 ?
3.1.1.29 N-acetyl-Leu-tRNA + H2O
-
 Fusarium culmorum N-acetyl-Leu + tRNA
-
 ?
3.1.1.29 N-acetyl-Lys-tRNA + H2O
-
 Fusarium culmorum N-acetyl-Lys + tRNA
-
 ?
3.1.1.29 N-acetyl-Met-tRNA + H2O
-
 Fusarium culmorum N-acetyl-Met + tRNA
-
 ?
3.1.1.29 N-acetyl-Phe-tRNA + H2O
-
 Fusarium culmorum N-acetyl-Phe + tRNA
-
 ?
3.1.1.29 N-acetyl-Ser-tRNA + H2O
-
 Fusarium culmorum N-acetyl-Ser + tRNA
-
 ?
3.1.1.29 N-acetyl-Tyr-tRNA + H2O
-
 Fusarium culmorum N-acetyl-Tyr + tRNA
-
 ?
3.1.1.29 N-acetyl-Val-tRNA + H2O
-
 Fusarium culmorum N-acetyl-Val + tRNA
-
 ?
3.1.1.29 Phe-tRNA + H2O
-
 Fusarium culmorum Phe + tRNA
-
 ?
3.1.1.29 Ser-tRNA + H2O
-
 Fusarium culmorum Ser + tRNA
-
 ?
3.1.1.29 Tyr-tRNA + H2O
-
 Fusarium culmorum Tyr + tRNA
-
 ?
3.1.1.29 Val-tRNA + H2O
-
 Fusarium culmorum Val + tRNA
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.29 45
-
 10 min, 70% loss of activity Fusarium culmorum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.29 7.5
-
-
 Fusarium culmorum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.1.29 6 8 30°C, 30 min, stable Fusarium culmorum