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Literature summary extracted from

  • Gallego, M.E.; Heredia, C.F.
    Purification and properties of an inducible aminoacyl-tRNA hydrolase from Artemia larvae (1982), Biochim. Biophys. Acta, 696, 57-65.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.29 deacetylated tRNA
-
 Artemia sp.
3.1.1.29 EDTA 5 mM, complete inhibition Artemia sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.29 Mg2+ the enzyme is maximally active in presence of divalent cations, Mg2+ or Mn2+. Maximal activity with 20 mM Mg2+ Artemia sp.
3.1.1.29 Mn2+ the enzyme is maximally active in presence of divalent cations, Mg2+ or Mn2+. Maximal activity with 2.5 mM, 50% of the activation with Mg2+ Artemia sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.29 55000
-
 gel filtration Artemia sp.

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.29 Artemia sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.29
-
 Artemia sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.29 larva
-
 Artemia sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.29 0.0149
-
-
 Artemia sp.

Storage Stability

EC Number Storage Stability Organism
3.1.1.29 -10°C, 50% glycerol, purified enzyme is stable for several months Artemia sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.29 Glu-tRNA + H2O
-
 Artemia sp. Glu + tRNA
-
 ?
3.1.1.29 Gly-tRNA + H2O
-
 Artemia sp. Gly + tRNA
-
 ?
3.1.1.29 Leu-tRNA + H2O
-
 Artemia sp. Leu + tRNA
-
 ?
3.1.1.29 Lys-tRNA + H2O
-
 Artemia sp. Lys + tRNA
-
 ?
3.1.1.29 N-acetyl-Glu-tRNA + H2O
-
 Artemia sp. N-acetyl-Glu + tRNA
-
 ?
3.1.1.29 N-acetyl-Leu-tRNA + H2O
-
 Artemia sp. N-acetyl-Leu + tRNA
-
 ?
3.1.1.29 N-acetyl-Lys-tRNA + H2O
-
 Artemia sp. N-acetyl-Lys + tRNA
-
 ?
3.1.1.29 N-acetyl-Phe-tRNA + H2O
-
 Artemia sp. N-acetyl-Phe + tRNA
-
 ?
3.1.1.29 N-acetyl-Ser-tRNA + H2O
-
 Artemia sp. N-acetyl-Ser + tRNA
-
 ?
3.1.1.29 N-acetyl-Val-tRNA + H2O
-
 Artemia sp. N-acetyl-Val + tRNA
-
 ?
3.1.1.29 Phe-tRNA + H2O
-
 Artemia sp. Phe + tRNA
-
 ?
3.1.1.29 Ser-tRNA + H2O
-
 Artemia sp. Ser + tRNA
-
 ?
3.1.1.29 Val-tRNA + H2O
-
 Artemia sp. Val + tRNA
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.29 50
-
 10 min, 85% loss of activity Artemia sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.29 7 7.5
-
 Artemia sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.1.29 6 7.5 4°C, 24 h, stable Artemia sp.