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Literature summary extracted from

  • Jost, J.P.; Bock, R.M.
    Enzymatic hydrolysis of N-substituted aminoacyl transfer ribonucleic acid in yeast (1969), J. Biol. Chem., 244, 5866-5873.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.1.29
-
 Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.29 EDTA 0.01 mM Saccharomyces cerevisiae
3.1.1.29 Mg2+ 0.1 mM Saccharomyces cerevisiae
3.1.1.29 tRNA uncharged yeast tRNA, competitive Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.29 0.000001
-
 N-acetyl-phenylalanyl-tRNA pH 7.0, 30°C Saccharomyces cerevisiae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.29 Mg2+ 0.001-0.01 MgCl2 activates Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.29 Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.29
-
 Saccharomyces cerevisiae

Storage Stability

EC Number Storage Stability Organism
3.1.1.29 -20°C, buffer solution: 0.05 M Tris-HCl, pH 7.0, 0.01 M 2-mercaptoethanol, 0.01 M magnesium acetate, or as an ammonium sulfate precipitate, 2 months without significant loss of activity Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.29 additional information N-acetyl-Val-adenosine is not a substrate, N-acetyl-Val-oligonucleotide is hydrolyzed very slowly. The specificity for the tRNA moiety does not seem to be directed towards a particular species of organism Saccharomyces cerevisiae ?
-
 ?
3.1.1.29 N-acetyl-Phe-tRNA + H2O enzyme with broad specificity Saccharomyces cerevisiae N-acetyl-Phe + tRNA
-
 ?
3.1.1.29 N-acetyl-Trp-tRNA + H2O
-
 Saccharomyces cerevisiae N-acetyl-Trp + tRNA
-
 ?
3.1.1.29 N-acetyl-Val-tRNA + H2O
-
 Saccharomyces cerevisiae N-acetyl-Val + tRNA
-
 ?
3.1.1.29 N-benzoyl-Gly-Gly-Phe-tRNA + H2O
-
 Saccharomyces cerevisiae N-benzoyl-Gly-Gly-Phe + tRNA
-
 ?
3.1.1.29 N-benzoyl-Gly-GlyGly-Phe-tRNA + H2O
-
 Saccharomyces cerevisiae N-benzoyl-Gly-Gly-Gly-Phe + tRNA
-
 ?
3.1.1.29 oligolysyl-tRNA + H2O
-
 Saccharomyces cerevisiae oligo-Lys + tRNA
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.29 7
-
 N-acetyl-Val-tRNA or N-acetyl-Trp as substrate Saccharomyces cerevisiae