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Literature summary extracted from

  • Mason, R.W.; Taylor, M.A.J.; Etherington, D.J.
    The purification and properties of cathepsin L from rabbit liver (1984), Biochem. J., 217, 209-217.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.22.15 benzyloxycarbonyl-Phe-Ala-CNH2
-
 Oryctolagus cuniculus
3.4.22.15 Benzyloxycarbonyl-Phe-Phe-CHN2
-
 Oryctolagus cuniculus
3.4.22.15 Ep-460
-
 Oryctolagus cuniculus
3.4.22.15 Ep-475
-
 Oryctolagus cuniculus
3.4.22.15 Hg2+ HgCl2; weak Oryctolagus cuniculus
3.4.22.15 iodoacetic acid
-
 Oryctolagus cuniculus
3.4.22.15 leupeptin
-
 Oryctolagus cuniculus
3.4.22.15 tosyl-Lys-CH2Cl
-
 Oryctolagus cuniculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.22.15 0.0007
-
 benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide
-
 Oryctolagus cuniculus
3.4.22.15 0.005
-
 succinyl-Ala-Phe-Lys-4-methylcoumarin 7-amide
-
 Oryctolagus cuniculus
3.4.22.15 0.006
-
 benzoyl-Phe-Val-Arg-4-methylcoumarin 7-amide
-
 Oryctolagus cuniculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.22.15 29000
-
 gel filtration Oryctolagus cuniculus

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.15 Oryctolagus cuniculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.22.15
-
 Oryctolagus cuniculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.22.15 liver
-
 Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.22.15 34.4
-
-
 Oryctolagus cuniculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.15 3-carboxypropionyl-Ala-Phe-Lys-4-methylcoumarin 7-amide + H2O
-
 Oryctolagus cuniculus ?
-
 ?
3.4.22.15 Arg-4-methylcoumarin 7-amide + H2O
-
 Oryctolagus cuniculus Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.22.15 azocasein + H2O
-
 Oryctolagus cuniculus ?
-
 ?
3.4.22.15 benzoyl-Phe-Val-Arg-4-methylcoumarin 7-amide + H2O
-
 Oryctolagus cuniculus ?
-
 ?
3.4.22.15 benzyloxycarbonyl-Arg-Arg-4-methylcoumarin 7-amide + H2O weak activity Oryctolagus cuniculus ?
-
 ?
3.4.22.15 benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide + H2O
-
 Oryctolagus cuniculus ?
-
 ?
3.4.22.15 Collagen + H2O
-
 Oryctolagus cuniculus ?
-
 ?
3.4.22.15 succinyl-Ala-Phe-Lys-4-methylcoumarin 7-amide + H2O
-
 Oryctolagus cuniculus ?
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.22.15 20
-
 benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide
-
 Oryctolagus cuniculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.22.15 3.3
-
 hydrolysis of collagen Oryctolagus cuniculus
3.4.22.15 5.2
-
 hydrolysis of azocasein Oryctolagus cuniculus
3.4.22.15 6
-
 hydrolysis of benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide Oryctolagus cuniculus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.22.15 3.3 3.8 about 60% of maximal activity at pH 3.0 and at pH 3.8, hydrolysis of collagen Oryctolagus cuniculus
3.4.22.15 4.5 6.5 pH 4.5: about 75% of maximal activity, pH 6.5: about 60% of maximal activity, hydrolysis of azocasein Oryctolagus cuniculus
3.4.22.15 5.5 6.5 pH 5.5: about 60% of maximal activity, pH 6.5: about 65% of maximal activity, hydrolysis of benzyloxycarbonyl-Phe-Arg-4-methycoumarin 7-amide Oryctolagus cuniculus