EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.4.25.2 | HslU | HslV can slowly hydrolyze insulin B-chain, casein or carboxymethylated lactalbumin, but its activity is stimulated 20fold by HslU in presence of ATP | Escherichia coli |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.4.25.2 | benzyloxycarbonyl-Ile-Glu(tert-butyl)-Ala-Leu-al | 0.001 mM, almost complete inhibition of peptidase activity, no inhibition of hydrolysis of insulin B-chain or other polypeptide substrates | Escherichia coli | |
3.4.25.2 | benzyloxycarbonyl-Leu-Leu-norvalinal | 0.004 mM, inhibits hydrolysis of both benzyloxycarbonyl-GGL-7-amido-4-methylcoumarin and insulin B-chain to a similar extent | Escherichia coli | |
3.4.25.2 | lactacystin | irreversible, much greater inhibition of the degradation of insulin B-chain than on peptide hydrolysis | Escherichia coli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.25.2 | 0.014 | - |
Insulin B-chain | pH 8, 37°C | Escherichia coli |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.25.2 | Escherichia coli | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.25.2 | benzyloxycarbonyl-GGL-7-amido-4-methylcoumarin + H2O | - |
Escherichia coli | benzyloxycarbonyl-GGL + 7-amino-4-methylcoumarin | - |
? | |
3.4.25.2 | carboxymethylated lactalbumin + H2O | - |
Escherichia coli | ? | - |
? | |
3.4.25.2 | casein + H2O | - |
Escherichia coli | ? | - |
? | |
3.4.25.2 | Insulin B-chain + H2O | - |
Escherichia coli | ? | - |
? | |
3.4.25.2 | additional information | no hydrolysis of gamma-globulin, lysozyme and bovine serum albumin | Escherichia coli | ? | - |
? |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
3.4.25.2 | ATP | HslV can slowly hydrolyze insulin B-chain, casein or carboxymethylated lactalbumin, but its activity is stimulated 20fold by HslU in presence of ATP | Escherichia coli |