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Literature summary extracted from

  • Yoo, S.J.; Seol, J.H.; Seong, I.S.; Kang, M.S.; Chung, C.H.
    ATP binding, but not its hydrolysis, is required for assembly and proteolytic activity of the HslVU protease in Escherichia coli (1997), Biochem. Biophys. Res. Commun., 238, 581-585.
    View publication on PubMed

Organism

EC Number Organism UniProt Comment Textmining
3.4.25.2 Escherichia coli
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.25.2 benzyloxycarbonyl-GGL-7-amido-4-methylcoumarin + H2O
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 Escherichia coli benzyloxycarbonyl-GGL + 7-amino-4-methylcoumarin
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3.4.25.2 Insulin B-chain + H2O HslVU degrades insulin B-chain even more rapidly in the presence of ATPgammaS than with ATP Escherichia coli ?
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3.4.25.2 additional information ATP-binding, but not its hydrolysis, is essential for assembly and proteolytic activity of HslVU Escherichia coli ?
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Subunits

EC Number Subunits Comment Organism
3.4.25.2 More HslVU is an ATP-dependent protease consisting of two multimeric components: the HslU ATPase and the HslV peptidase Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
3.4.25.2 adenosine 5'-(alpha,beta-methylene)triphosphate HslVU degrades insulin B-chain more rapidly in the presence of ATPgammaS than with ATP Escherichia coli
3.4.25.2 ATP ATP-binding, but not its hydrolysis, is essential for assembly and proteolytic activity of HslVU. The ability of ATP and its analogs in supporting the proteolytic activity is closely correlated with their ability in supporting the oligomerization of HslU and the formation of the HslVU complex Escherichia coli
3.4.25.2 ATPgammaS HslVU degrades insulin B-chain more rapidly in the presence of ATPgammaS than with ATP Escherichia coli
3.4.25.2 beta,gamma-Imido-ATP supports proteolytic activity to an extent less than 10% of that seen with ATP Escherichia coli