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Literature summary extracted from
- Bovine leukemia virus: purification and characterization of the aspartic protease (1993), Virology, 193, 680-689.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.23.B9 | YDPPAI-statine-II | - |
bovine leukemia virus |  |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.23.B9 | (NH4)2SO4 | with YDPPAILPII as substrate, most active at 1-2 M NaCl or (NH4)2SO4 | bovine leukemia virus | |
| 3.4.23.B9 | NaCl | with YDPPAILPII as substrate, most active at 1-2 M NaCl or (NH4)2SO4. With human T-cell leukemia virus recombinant gag precursor activity is higher at 0.5 M NaCl | bovine leukemia virus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.23.B9 | 14000 | - |
2 * 14000, SDS-PAGE | bovine leukemia virus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.23.B9 | bovine leukemia virus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.23.B9 | - |
bovine leukemia virus |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.4.23.B9 | 0.25 | - |
reaction with YDPPAILPII | bovine leukemia virus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.23.B9 | YDPPAILPII + H2O | best substrate, bearing the natural cleavage site between the matrix and the capsid proteins of BLV Gag precursor polyprotein | bovine leukemia virus | YDPPAIL + PII | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.4.23.B9 | ? | 2 * 14000, SDS-PAGE | bovine leukemia virus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.23.B9 | 40 | - |
reaction with YDPPAILPII | bovine leukemia virus |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.23.B9 | 20 | 80 | 20°C: about 45% of maximal activity, 80°C: about 70% of maximal activity, reaction with YDPPAILPII | bovine leukemia virus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.23.B9 | 5 | 6 | reaction with YDPPAILPII | bovine leukemia virus |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.23.B9 | 3.5 | 7.5 | pH 3.5: about 90% of maximal activity, pH 7.5: about 70% of maximal activity, reaction with YDPPAILPII | bovine leukemia virus |
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