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Literature summary extracted from

  • Menendez-Arias, L.; Weber, I.T.; Oroszlan, S.
    Mutational analysis of the substrate binding pocket of murine leukemia virus protease and comparison with human immunodeficiency virus proteases (1995), J. Biol. Chem., 270, 29162-29168.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.23.B5 expression in Escherichia coli, wild-type and mutant enzymes are expressed in fusion with the Schistosoma japonicum glutathione S-transferase Moloney murine leukemia virus

Protein Variants

EC Number Protein Variants Comment Organism
3.4.23.B5 A57I mutation has a strong influence on substrate specificity Moloney murine leukemia virus
3.4.23.B5 C88T mutant enzyme shows an increased preference for hydrophobic amino acids at P4 and P2 position in a series of VSQNYPIVQ analogs Moloney murine leukemia virus
3.4.23.B5 E15R mutant enzyme shows similar kinetic parameters to that of the mutant enzyme Moloney murine leukemia virus
3.4.23.B5 G60F mutant enzyme shows an increased preference for hydrophobic amino acids at P4 and P2 position in a series of VSQNYPIVQ analogs Moloney murine leukemia virus
3.4.23.B5 H37D mutation has a strong influence on substrate specificity Moloney murine leukemia virus
3.4.23.B5 L92I mutant enzyme shows an increased preference for hydrophobic amino acids at P4 and P2 position in a series of VSQNYPIVQ analogs Moloney murine leukemia virus
3.4.23.B5 L92I mutation has a strong influence on substrate specificity Moloney murine leukemia virus
3.4.23.B5 V39I mutant enzyme shows similar kinetic parameters to that of the mutant enzyme Moloney murine leukemia virus
3.4.23.B5 V39I mutation has a strong influence on substrate specificity Moloney murine leukemia virus
3.4.23.B5 V39I/V54I mutant enzyme shows similar kinetic parameters to that of the mutant enzyme Moloney murine leukemia virus
3.4.23.B5 V54I mutant enzyme shows similar kinetic parameters to that of the mutant enzyme Moloney murine leukemia virus
3.4.23.B5 V54I mutant enzyme shows an increased preference for hydrophobic amino acids at P4 and P2 position in a series of VSQNYPIVQ analogs Moloney murine leukemia virus
3.4.23.B5 W53I mutant enzyme shows an increased preference for hydrophobic amino acids at P4 and P2 position in a series of VSQNYPIVQ analogs Moloney murine leukemia virus
3.4.23.B5 W53I/Q55G mutant enzyme does not cleave the wild-type substrates: VSQNYPIVQ, VIQNYPIVQ and VSQNYPLVQ Moloney murine leukemia virus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.23.B5 0.01
-
 VSQNYPLVQ pH 6.0, 37°C, mutant enzyme V54I Moloney murine leukemia virus
3.4.23.B5 0.019
-
 VSQNYPLVQ pH 6.0, 37°C, wild-type enzyme Moloney murine leukemia virus
3.4.23.B5 0.029
-
 VSQNYPLVQ pH 6.0, 37°C, mutant enzyme A57I Moloney murine leukemia virus
3.4.23.B5 0.036
-
 VIQNYPIVQ pH 6.0, 37°C, mutant enzyme A57I Moloney murine leukemia virus
3.4.23.B5 0.039
-
 VIQNYPIVQ pH 6.0, 37°C, wild-type enzyme Moloney murine leukemia virus
3.4.23.B5 0.041
-
 VSQNYPIVQ pH 6.0, 37°C, mutant enzyme H37D Moloney murine leukemia virus
3.4.23.B5 0.049
-
 VSQNYPLVQ pH 6.0, 37°C, mutant enzyme V39I Moloney murine leukemia virus
3.4.23.B5 0.052
-
 VSQNYPLVQ pH 6.0, 37°C, mutant enzyme V39I/V54I and V39I/A57I Moloney murine leukemia virus
3.4.23.B5 0.061
-
 VIQNYPIVQ pH 6.0, 37°C, mutant enzyme V39I/A57I Moloney murine leukemia virus
3.4.23.B5 0.074
-
 VSQNYPLVQ pH 6.0, 37°C, mutant enzyme L92I Moloney murine leukemia virus
3.4.23.B5 0.134
-
 VIQNYPIVQ pH 6.0, 37°C, mutant enzyme V39I Moloney murine leukemia virus
3.4.23.B5 0.174
-
 VIQNYPIVQ pH 6.0, 37°C, mutant enzyme L92I Moloney murine leukemia virus
3.4.23.B5 0.234
-
 VSQNYPIVQ pH 6.0, 37°C, mutant enzyme V54I Moloney murine leukemia virus
3.4.23.B5 0.261
-
 VSQNYPIVQ pH 6.0, 37°C, wild-type enzyme Moloney murine leukemia virus
3.4.23.B5 0.265
-
 VSQNYPIVQ pH 6.0, 37°C, mutant enzyme E15R Moloney murine leukemia virus
3.4.23.B5 0.268
-
 VSQNYPIVQ pH 6.0, 37°C, mutant enzyme V39I Moloney murine leukemia virus
3.4.23.B5 0.287
-
 VSQNYPIVQ pH 6.0, 37°C, mutant enzyme V39I/V54I Moloney murine leukemia virus
3.4.23.B5 0.29
-
 VSQNYPIVQ pH 6.0, 37°C, mutant enzyme Y63V Moloney murine leukemia virus
3.4.23.B5 0.356
-
 VSQNYPIVQ pH 6.0, 37°C, mutant enzyme A57I Moloney murine leukemia virus
3.4.23.B5 0.456
-
 VSQNYPIVQ pH 6.0, 37°C, mutant enzyme V39I/A57I Moloney murine leukemia virus
3.4.23.B5 1.238
-
 VSQNYPIVQ pH 6.0, 37°C, mutant enzyme L92I Moloney murine leukemia virus
3.4.23.B5 2.046
-
 VSQNYPIVQ pH 6.0, 37°C, mutant enzyme W53I Moloney murine leukemia virus

Organism

EC Number Organism UniProt Comment Textmining
3.4.23.B5 Moloney murine leukemia virus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.23.B5 VIQNYPIVQ + H2O
-
 Moloney murine leukemia virus VIQNY + PIVQ
-
 ?
3.4.23.B5 VSQNYPIVQ + H2O
-
 Moloney murine leukemia virus VSQNY + PIVQ
-
 ?
3.4.23.B5 VSQNYPLVQ + H2O
-
 Moloney murine leukemia virus VSQNY + PLVQ
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.23.B5 0.003
-
 VSQNYPLVQ pH 6.0, 37°C, mutant enzyme V39I/A57I Moloney murine leukemia virus
3.4.23.B5 0.012
-
 VIQNYPIVQ pH 6.0, 37°C, mutant enzyme H37D Moloney murine leukemia virus
3.4.23.B5 0.023
-
 VSQNYPLVQ pH 6.0, 37°C, mutant enzyme H37D Moloney murine leukemia virus
3.4.23.B5 0.0442
-
 VSQNYPIVQ pH 6.0, 37°C, mutant enzyme L92I Moloney murine leukemia virus
3.4.23.B5 0.062
-
 VSQNYPLVQ pH 6.0, 37°C, mutant enzyme A57I Moloney murine leukemia virus
3.4.23.B5 0.065
-
 VIQNYPIVQ pH 6.0, 37°C, mutant enzyme V39I/A57I Moloney murine leukemia virus
3.4.23.B5 0.072
-
 VSQNYPIVQ pH 6.0, 37°C, mutant enzyme V39I/A57I Moloney murine leukemia virus
3.4.23.B5 0.075
-
 VSQNYPIVQ pH 6.0, 37°C, mutant enzyme H37D Moloney murine leukemia virus
3.4.23.B5 0.11
-
 VSQNYPLVQ pH 6.0, 37°C, mutant enzyme V39I/V54I Moloney murine leukemia virus
3.4.23.B5 0.12
-
 VIQNYPIVQ pH 6.0, 37°C, mutant enzyme L92I Moloney murine leukemia virus
3.4.23.B5 0.2
-
 VSQNYPIVQ pH 6.0, 37°C, mutant enzyme Y63V Moloney murine leukemia virus
3.4.23.B5 0.2
-
 VSQNYPLVQ pH 6.0, 37°C, mutant enzyme V39I Moloney murine leukemia virus
3.4.23.B5 0.22
-
 VIQNYPIVQ pH 6.0, 37°C, mutant enzyme V39I Moloney murine leukemia virus
3.4.23.B5 0.23
-
 VSQNYPIVQ pH 6.0, 37°C, mutant enzyme A57I Moloney murine leukemia virus
3.4.23.B5 0.24
-
 VSQNYPLVQ pH 6.0, 37°C, wild-type enzyme Moloney murine leukemia virus
3.4.23.B5 0.3
-
 VSQNYPIVQ pH 6.0, 37°C, mutant enzymes E15R and W53I Moloney murine leukemia virus
3.4.23.B5 0.34
-
 VSQNYPLVQ pH 6.0, 37°C, mutant enzyme L92I Moloney murine leukemia virus
3.4.23.B5 0.35
-
 VSQNYPIVQ pH 6.0, 37°C, mutant enzyme V54I Moloney murine leukemia virus
3.4.23.B5 0.4
-
 VIQNYPIVQ pH 6.0, 37°C, mutant enzyme A57I Moloney murine leukemia virus
3.4.23.B5 0.46
-
 VSQNYPIVQ pH 6.0, 37°C, mutant enzyme V39I/V54I Moloney murine leukemia virus
3.4.23.B5 0.48
-
 VIQNYPIVQ pH 6.0, 37°C, wild-type enzyme Moloney murine leukemia virus
3.4.23.B5 0.55
-
 VSQNYPIVQ pH 6.0, 37°C, wild-type enzyme Moloney murine leukemia virus
3.4.23.B5 0.74
-
 VSQNYPIVQ pH 6.0, 37°C, mutant enzyme V39I Moloney murine leukemia virus
3.4.23.B5 1.9
-
 VSQNYPLVQ pH 6.0, 37°C, mutant enzyme V54I Moloney murine leukemia virus