Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Väisanen, V.; Lovgren, J.; Hellman, J.; Piironen, T.; Lilja, H.; Pettersson, K.
    Characterization and processing of prostate specific antigen (hK3) and human glandular kallikrein (hK2) secreted by LNCaP cells (1999), Prostate Cancer Prostatic. Dis., 2, 91-97.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.21.77 additional information inactive proPSA enzyme can be activated by recombinant kallikrein 2 to the mature form Homo sapiens

Application

EC Number Application Comment Organism
3.4.21.77 medicine the size of the fraction of free enzyme in serum is a marker to distinguish benign hyperplasia from malignant carcinoma Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.77 serum inhibits the mature enzyme Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.21.77 extracellular secretion Homo sapiens
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.77 28110 30000 inactive zymogen peak 2, gel filtration, SDS-PAGE and mass spectrometry Homo sapiens
3.4.21.77 28880 30000 active mature enzyme, gel filtration and mass spectrometry Homo sapiens
3.4.21.77 87000
-
inactive zymogen peak 1, gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.21.77 additional information Homo sapiens enzyme can be activated by recombinant kallikrein 2 ?
-
?
3.4.21.77 polypeptide + H2O Homo sapiens
-
peptides
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.77 Homo sapiens
-
2 isozyme of the zymogen with different pI, several isoforms with different pIs of the mature enzyme
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.21.77 proteolytic modification enzyme can be activated by recombinant kallikrein 2 Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.77 from LNCaP cell culture medium Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.21.77 prostate
-
Homo sapiens
-
3.4.21.77 prostate cancer cell very low content of free enzyme Homo sapiens
-
3.4.21.77 prostate cancer cell produces equal amounts of zymogen proPSA and one-chain mature enzyme form, no intermediate forms Homo sapiens
-
3.4.21.77 prostate cancer cell metastatic prostate adenocarcinoma cell line LNCaP Homo sapiens
-
3.4.21.77 serum enzyme is mainly complexed to alpha1-antichymotrypsin, i.e. ACT, a small part of free enzyme Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.77 KGISSQY-7-amido-4-methylcoumarin + H2O fluorogenic substrate Homo sapiens KGISSQY + 7-amino-4-methylcoumarin
-
?
3.4.21.77 additional information enzyme can be activated by recombinant kallikrein 2 Homo sapiens ?
-
?
3.4.21.77 PFR-7-amido-4-methylcoumarin + H2O fluorogenic substrate Homo sapiens PFR + 7-amino-4-methylcoumarin
-
?
3.4.21.77 polypeptide + H2O
-
Homo sapiens peptides
-
?

Subunits

EC Number Subunits Comment Organism
3.4.21.77 More active mature enzyme consists of only 1 chain Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
3.4.21.77 K3
-
Homo sapiens
3.4.21.77 prostate specific antigen
-
Homo sapiens
3.4.21.77 prostate-specific antigen
-
Homo sapiens

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.4.21.77 Homo sapiens several isozymes of active mature enzyme
-
6.4
3.4.21.77 Homo sapiens 2 enzyme proforms with different pI: pH 8.2 and pH 8.4
-
8.2
3.4.21.77 Homo sapiens 2 enzyme proforms with different pI: pH 8.2 and pH 8.4
-
8.4