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Literature summary extracted from

  • Charlesworth, M.C.; Young, C.Y.; Miller, V.M.; Tindall, D.J.
    Kininogenase activity of prostate-derived human glandular kallikrein (hK2) purified from seminal fluid (1999), J. Androl., 20, 220-229.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.35 Protein C inhibitor covalent complex formation with K2 Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.35 32000
-
 x * 32000, K2, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.21.35 kininogen + H2O Homo sapiens important role in bradykinin release in seminal fluid bradykinin + kinin heavy chain + kinin light chain
-
 ?
3.4.21.35 polypeptide + H2O Homo sapiens
-
 peptides
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.35 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.35 glandular kallikrein, 5000fold, to homogeneity Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.21.35 prostate gland prostate-specific Homo sapiens
-
3.4.21.35 semen
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.21.35 additional information
-
-
 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.35 H-D-Pro-Phe-Arg-7-amido-4-methylcoumarin + H2O i.e. S-2302 Homo sapiens H-D-Pro-Phe-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.21.35 kininogen + H2O important role in bradykinin release in seminal fluid Homo sapiens bradykinin + kinin heavy chain + kinin light chain
-
 ?
3.4.21.35 kininogen + H2O high-molecular weight kininogen Homo sapiens bradykinin + kinin
-
 ?
3.4.21.35 kininogen + H2O 3 additional claevage sites at Arg427, Arg437, and Arg457 Homo sapiens bradykinin + kinin
-
 ?
3.4.21.35 polypeptide + H2O
-
 Homo sapiens peptides
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.21.35 ? x * 32000, K2, SDS-PAGE Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.21.35 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.21.35 8.2
-
 assay at Homo sapiens