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Literature summary extracted from

  • Fiedler, F.
    Pig pancreatic kallikrein A and B (1976), Methods Enzymol., 45, 289-303.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.35 cinnamoyl imidazole
-
 Sus scrofa
3.4.21.35 diisopropyl fluorophosphate
-
 Sus scrofa
3.4.21.35 indolacryloyl imidazole
-
 Sus scrofa
3.4.21.35 p-nitrophenyl p'-guanidinobenzoate
-
 Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.35 0.031
-
 N-alpha-toluenesulfonyl-L-Arg methyl ester pH 8.0, 40°C Sus scrofa
3.4.21.35 0.104
-
 N-alpha-benzoyl-L-Arg ethyl ester pH 8.0, 40°C Sus scrofa
3.4.21.35 0.125
-
 N-alpha-benzoyl-L-Arg methyl ester pH 8.0, 40°C Sus scrofa
3.4.21.35 2
-
 N-alpha-benzoyl-L-Lys methyl ester pH 8.0, 40°C Sus scrofa
3.4.21.35 2.44
-
 N-alpha-benzoyl-L-Orn methyl ester pH 8.0, 40°C Sus scrofa
3.4.21.35 12.3
-
 N-alpha-benzoyl-L-citrulline methyl ester pH 8.0, 40°C Sus scrofa
3.4.21.35 50
-
 N-alpha-acetyl-Tyr ethyl ester pH 8.0, 40°C Sus scrofa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.35 31000 35000 gel filtration Sus scrofa
3.4.21.35 33500
-
 light scattering Sus scrofa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.21.35 polypeptide + H2O Sus scrofa
-
 peptides
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.35 Sus scrofa
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.21.35 side-chain modification kallikrein A contains 5.6% carbohydrate by weight, kallikrein B contains 11.5% carbohydrate per weight. Carbohydrate is located on both chains of kallikrein B Sus scrofa

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.35 kallikrein A and B Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.21.35 pancreas
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.21.35 additional information
-
-
 Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.35 N-alpha-acetyl-Tyr ethyl ester + H2O
-
 Sus scrofa N-alpha-acetyl-Tyr + ethanol
-
 ?
3.4.21.35 N-alpha-benzoyl-L-Arg ethyl ester + H2O
-
 Sus scrofa N-alpha-benzoyl-L-Arg + ethanol
-
 ?
3.4.21.35 N-alpha-benzoyl-L-Arg methyl ester + H2O
-
 Sus scrofa N-alpha-benzoyl-L-Arg + methanol
-
 ?
3.4.21.35 N-alpha-benzoyl-L-citrulline methyl ester + H2O
-
 Sus scrofa N-alpha-benzoyl-L-citrulline + methanol
-
 ?
3.4.21.35 N-alpha-benzoyl-L-Lys methyl ester + H2O
-
 Sus scrofa N-alpha-benzoyl-L-Lys + methanol
-
 ?
3.4.21.35 N-alpha-benzoyl-L-Orn methyl ester + H2O
-
 Sus scrofa N-alpha-benzoyl-L-Orn + methanol
-
 ?
3.4.21.35 polypeptide + H2O
-
 Sus scrofa peptides
-
 ?
3.4.21.35 Tosyl-Arg methyl ester + H2O
-
 Sus scrofa Tosyl-Arg + methanol
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.21.35 1.8
-
 N-alpha-benzoyl-L-Orn methyl ester pH 8.0, 25°C Sus scrofa
3.4.21.35 2.7
-
 N-alpha-toluenesulfonyl-L-Arg methyl ester pH 8.0, 25°C Sus scrofa
3.4.21.35 11.2
-
 N-alpha-acetyl-Tyr ethyl ester pH 8.0, 25°C Sus scrofa
3.4.21.35 15.5
-
 N-alpha-benzoyl-L-Lys methyl ester pH 8.0, 25°C Sus scrofa
3.4.21.35 86.4
-
 N-alpha-benzoyl-L-citrulline methyl ester pH 8.0, 25°C Sus scrofa
3.4.21.35 123
-
 N-alpha-benzoyl-L-Arg ethyl ester pH 8.0, 25°C Sus scrofa
3.4.21.35 154
-
 N-alpha-benzoyl-L-Arg methyl ester pH 8.0, 25°C Sus scrofa