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Literature summary extracted from

  • Scott, C.J.; McDowell, A.; Martin, S.L.; Lynas, J.F.; Vandenbroeck, K.; Walker, B.
    Irreversible inhibition of the bacterial cysteine protease-transpeptidase sortase (SrtA) by substrate-derived affinity labels (2002), Biochem. J., 366, 953-958.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
3.4.22.70 medicine substrate-derived irreversible inhibitors of SrtA that might find application in delineating the role of the cysteine protease-transpeptidase in cell surface protein sorting and adherence of Gram-positive organisms Staphylococcus aureus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.22.70 expression in Escherichia coli Staphylococcus aureus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.22.70 benzyloxycarbonyl-Leu-Pro-Ala-Thr-CH2Cl irreversible inhibitor of recombinant enzyme Staphylococcus aureus
3.4.22.70 benzyloxycarbonyl-Leu-Pro-Ala-Thr-CHN2 irreversible inhibitor of recombinant enzyme Staphylococcus aureus

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.70 Staphylococcus aureus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.70 4-[[4'-(dimethylamino)phenyl]azo]-benzoyl-Gln-Ala-Leu-Pro-Glu-Thr-Gly-Glu-Glu-5-[(2'-aminoethyl)-amino]naphthalenesulfonic acid + H2O
-
 Staphylococcus aureus 4-[[4'-(dimethylamino)phenyl]azo]-benzoyl-Gln-Ala-Leu-Pro-Glu-Thr + Gly-Glu-Glu-5-[(2'-aminoethyl)-amino]naphthalenesulfonic acid
-
 ?

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.4.22.70 0.00000021
-
 benzyloxycarbonyl-Leu-Pro-Ala-Thr-CH2Cl pH 7.5, 37°C Staphylococcus aureus
3.4.22.70 0.00000022
-
 benzyloxycarbonyl-Leu-Pro-Ala-Thr-CHN2 pH 7.5, 37°C Staphylococcus aureus