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Literature summary extracted from

  • Timm, D.E.
    The crystal structure of the mouse glandular kallikrein-13 (prorenin converting enzyme) (1997), Protein Sci., 6, 1418-1425.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.4.21.119 both hanging and sitting drop vapour diffusion method, protein solution: 57 mg/ml, precipitant solution: 10-15% PEG 8000, 200 mM Li2SO4, 100 mM sodium cacodylate, pH 6.5, room temperature, X-ray diffraction structure determination and analysis Mus musculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.119 27000
-
 2 * 27000, SDS-PAGE Mus musculus
3.4.21.119 54000
-
 about, gel filtration and crystal structure Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.21.119 polypeptide + H2O Mus musculus
-
 peptides
-
 ?
3.4.21.119 Prorenin + H2O Mus musculus inactive precursor Renin + ? product is an active enzyme ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.119 Mus musculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.119
-
 Mus musculus

Reaction

EC Number Reaction Comment Organism Reaction ID
3.4.21.119 Hydrolyses mouse Ren2 protein (a species of prorenin present in the submandibular gland) on the carboxy side of the arginine residue at the Lys-Arg-/- pair in the N-terminus, to yield mature renin substrate binding Mus musculus
a
3.4.21.119 Hydrolyses mouse Ren2 protein (a species of prorenin present in the submandibular gland) on the carboxy side of the arginine residue at the Lys-Arg-/- pair in the N-terminus, to yield mature renin enzyme is a serine protease Mus musculus
a
3.4.21.119 Hydrolyses mouse Ren2 protein (a species of prorenin present in the submandibular gland) on the carboxy side of the arginine residue at the Lys-Arg-/- pair in the N-terminus, to yield mature renin active site is located in the cleft between two beta-barrel domains, with an ordered kallikrein loop conformation close to it Mus musculus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.21.119 submandibular gland
-
 Mus musculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.119 polypeptide + H2O
-
 Mus musculus peptides
-
 ?
3.4.21.119 Prorenin + H2O cleavage site at paired basic amino acid residues Mus musculus Renin + ?
-
 ?
3.4.21.119 Prorenin + H2O mouse prorenin from submandibular gland Mus musculus Renin + ?
-
 ?
3.4.21.119 Prorenin + H2O inactive precursor Mus musculus Renin + ? product is an active enzyme ?

Subunits

EC Number Subunits Comment Organism
3.4.21.119 dimer 2 * 27000, SDS-PAGE Mus musculus
3.4.21.119 More enzyme contains 2 disulfide bridges at cystines 22-157 and 136-201 Mus musculus

Synonyms

EC Number Synonyms Comment Organism
3.4.21.119 GK-13
-
 Mus musculus
3.4.21.119 glandular kallikrein 13
-
 Mus musculus
3.4.21.119 prorenin converting enzyme
-
 Mus musculus
3.4.21.119 prorenin-converting enzyme
-
 Mus musculus