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Literature summary extracted from
- A novel isoform of a kallikrein-like protease, TLSP/hippostasin, (PRSS20), is expressed in the human brain and prostate (2000), Biochem. Biophys. Res. Commun., 272, 205-211.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.4.21.B42 | gene KLK11, identical to gene TLSP or PRSS20, splicing variant 1, prostate-type isozyme, DNA sequence determination and analysis, transient expression of Myc-His-tagged enzyme in COS-7 cells, expression as fusion protein with trypsinogen signal peptide followed by a His-tag in insect cells via baculovirus infection | Homo sapiens |
| 3.4.21.B42 | gene KLK11, splicing variant 2, brain-type isozyme, DNA sequence determination and analysis, transient expression of Myc-His-tagged enzyme in COS-7 cells, expression as fusion protein with trypsinogen signal peptide followed by a His-tag in insect cells via baculovirus infection | Homo sapiens |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.4.21.B42 | extracellular | secretion into the medium | Homo sapiens | - |
- |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.B42 | 40000 | - |
x * 40000, SDS-PAGE | Homo sapiens |
| 3.4.21.B42 | 44000 | - |
x * 44000, SDS-PAGE | Homo sapiens |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.21.B42 | polypeptide + H2O | Homo sapiens | - |
peptides | - |
? |  |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.21.B42 | Homo sapiens | Q9UBX7 | prostate-type isozyme | - |
| 3.4.21.B42 | Homo sapiens | Q9UBX7 | splicing variant 1 | - |
| 3.4.21.B42 | Homo sapiens | Q9UBX7 | brain-type isozyme | - |
| 3.4.21.B42 | Homo sapiens | Q9UBX7 | splicing variant 2 | - |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.4.21.B42 | glycoprotein | 4 possible glycosylation sites | Homo sapiens |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 3.4.21.B42 | proteolytic cleavage of polypeptides, preferential cleavage site: Arg- | enzyme is trypsin-like a serine protease | Homo sapiens |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.4.21.B42 | brain | brain-type isozyme, secreted into the medium | Homo sapiens | - |
| 3.4.21.B42 | epithelium | prostate | Homo sapiens | - |
| 3.4.21.B42 | heart | brain-type isozyme | Homo sapiens | - |
| 3.4.21.B42 | hippocampus | especially expressed in pyramidal neurons | Homo sapiens | - |
| 3.4.21.B42 | lung | prostate-type isozyme | Homo sapiens | - |
| 3.4.21.B42 | neuron | pyramidal, hippocampus | Homo sapiens | - |
| 3.4.21.B42 | prostate | especially expressed in secretory epithelial cells | Homo sapiens | - |
| 3.4.21.B42 | prostate | prostate-type isozyme, secreted into the medium | Homo sapiens | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.21.B42 | acetyl-Arg-Arg-4-methyl-coumaryl-7-amide + H2O | recombinant enzyme | Homo sapiens | acetyl-Arg-Arg + 7-amino-4-methylcoumarin | - |
? | |
| 3.4.21.B42 | acetyl-Phe-Arg-4-methyl-coumaryl-7-amide + H2O | best substrate | Homo sapiens | acetyl-Phe-Arg + 7-amino-4-methylcoumarin | - |
? | |
| 3.4.21.B42 | acetyl-Phe-Arg-4-methyl-coumaryl-7-amide + H2O | recombinant enzyme | Homo sapiens | acetyl-Phe-Arg + 7-amino-4-methylcoumarin | - |
? | |
| 3.4.21.B42 | Arg-4-methyl-coumaryl-7-amide + H2O | recombinant enzyme | Homo sapiens | Arg + 7-amino-4-methylcoumarin | - |
? | |
| 3.4.21.B42 | benzyl-Arg-4-methyl-coumaryl-7-amide + H2O | recombinant enzyme | Homo sapiens | benzyl-Arg + 7-amino-4-methylcoumarin | - |
? | |
| 3.4.21.B42 | benzyl-Phe-Arg-4-methyl-coumaryl-7-amide + H2O | recombinant enzyme | Homo sapiens | benzyl-Phe-Arg + 7-amino-4-methylcoumarin | - |
? | |
| 3.4.21.B42 | benzyloxycarbonyl-Gln-Ala-Arg-4-methyl-coumaryl-7-amide + H2O | recombinant enzyme | Homo sapiens | benzyloxycarbonyl-Gln-Ala-Arg + 7-amino-4-methylcoumarin | - |
? | |
| 3.4.21.B42 | benzyloxycarbonyl-Phe-Ser-Arg-4-methyl-coumaryl-7-amide + H2O | recombinant enzyme | Homo sapiens | benzyloxycarbonyl-Phe-Ser-Arg + 7-amino-4-methylcoumarin | - |
? | |
| 3.4.21.B42 | benzyloxycarbonyl-Val-Leu-Lys-4-methyl-coumaryl-7-amide + H2O | recombinant enzyme | Homo sapiens | benzyloxycarbonyl-Val-Leu-Lys + 7-amino-4-methylcoumarin | - |
? | |
| 3.4.21.B42 | benzyloxycarbonyl-Val-Pro-Arg-4-methyl-coumaryl-7-amide + H2O | recombinant enzyme | Homo sapiens | benzyloxycarbonyl-Val-Pro-Arg + 7-amino-4-methylcoumarin | - |
? | |
| 3.4.21.B42 | polypeptide + H2O | - |
Homo sapiens | peptides | - |
? | |
| 3.4.21.B42 | Pro-Phe-Arg-4-methyl-coumaryl-7-amide + H2O | recombinant enzyme | Homo sapiens | Pro-Phe-Arg + 7-amino-4-methylcoumarin | - |
? | |
| 3.4.21.B42 | Pyr-Gly-Arg-4-methyl-coumaryl-7-amide + H2O | recombinant enzyme | Homo sapiens | Pyr-Gly-Arg + 7-amino-4-methylcoumarin | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.4.21.B42 | ? | x * 40000, SDS-PAGE | Homo sapiens |
| 3.4.21.B42 | ? | x * 44000, SDS-PAGE | Homo sapiens |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.4.21.B42 | kallikrein-like protease | - |
Homo sapiens |
| 3.4.21.B42 | TLSP | - |
Homo sapiens |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.B42 | 8 | - |
assay at | Homo sapiens |
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