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Literature summary extracted from

  • Kikkawa, Y.; Yamanaka, N.; Tada, J.; Kanamori, N.; Tsumura, K.; Hosoi, K.
    Prorenin processing and restricted endoproteolysis by mouse tissue kallikrein family enzymes (mK1, mK9, mK13, and mK22) (1998), Biochim. Biophys. Acta, 1382, 55-64.
    View publication on PubMed

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.21.B40 polypeptide + H2O Mus musculus
-
 peptides
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.B40 Mus musculus
-
 8-10 weeks old male ICR mice
-
3.4.21.119 Mus musculus
-
 8-10 weeks old male ICR mice
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.B40
-
 Mus musculus
3.4.21.119
-
 Mus musculus

Reaction

EC Number Reaction Comment Organism Reaction ID
3.4.21.B40 proteolytic cleavage of polypeptides, preferential cleavage site at paired basic amino acid residues preference for Xaa-Arg-Arg- motif Mus musculus
a
3.4.21.119 Hydrolyses mouse Ren2 protein (a species of prorenin present in the submandibular gland) on the carboxy side of the arginine residue at the Lys-Arg-/- pair in the N-terminus, to yield mature renin preference for Xaa-Arg-Arg- motif Mus musculus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.21.B40 submandibular gland
-
 Mus musculus
-
3.4.21.119 submandibular gland
-
 Mus musculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.21.B40 0.0005
-
 purified enzyme, substrate Pyr-Arg-Tyr-Lys-Arg-4-methyl-coumaryl-7-amide Mus musculus
3.4.21.119 0.0011
-
 purified enzyme, substrate benzyloxycarbonyl-Gln-Arg-Arg-4-methyl-coumaryl-7-amide Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.B40 benzyloxycarbonyl-Arg-Val-Arg-Arg-4-methyl-coumaryl-7-amide + H2O low activity Mus musculus benzyloxycarbonyl-Arg-Val-Arg-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.21.B40 benzyloxycarbonyl-Gln-Arg-Arg-4-methyl-coumaryl-7-amide + H2O
-
 Mus musculus benzyloxycarbonyl-Gln-Arg-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.21.B40 benzyloxycarbonyl-Glu-Lys-Lys-4-methyl-coumaryl-7-amide + H2O low activity Mus musculus benzyloxycarbonyl-Glu-Lys-Lys + 7-amino-4-methylcoumarin
-
 ?
3.4.21.B40 benzyloxycarbonyl-Leu-Lys-Arg-4-methyl-coumaryl-7-amide + H2O
-
 Mus musculus benzyloxycarbonyl-Leu-Lys-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.21.B40 polypeptide + H2O
-
 Mus musculus peptides
-
 ?
3.4.21.B40 prorenin 2 + H2O recombinant substrate, enzymatically inactive proform Mus musculus renin 2 + ? product is the enzymatically active enzyme form ?
3.4.21.B40 prorenin 2 + H2O specific for mouse prorenin 2 from submandibular gland Mus musculus renin 2 + ? product is the enzymatically active enzyme form ?
3.4.21.B40 Pyr-Arg-Tyr-Lys-Arg-4-methyl-coumaryl-7-amide + H2O best substrate Mus musculus Pyr-Arg-Tyr-Lys-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.21.119 benzyloxycarbonyl-Arg-Val-Arg-Arg-4-methyl-coumaryl-7-amide + H2O low activity Mus musculus benzyloxycarbonyl-Arg-Val-Arg-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.21.119 benzyloxycarbonyl-Gln-Arg-Arg-4-methyl-coumaryl-7-amide + H2O best substrate Mus musculus benzyloxycarbonyl-Gln-Arg-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.21.119 benzyloxycarbonyl-Glu-Lys-Lys-4-methyl-coumaryl-7-amide + H2O low activity Mus musculus benzyloxycarbonyl-Glu-Lys-Lys + 7-amino-4-methylcoumarin
-
 ?
3.4.21.119 benzyloxycarbonyl-Leu-Lys-Arg-4-methyl-coumaryl-7-amide + H2O
-
 Mus musculus benzyloxycarbonyl-Leu-Lys-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.21.119 Prorenin + H2O cleavage site at paired basic amino acid residues Mus musculus Renin + ?
-
 ?
3.4.21.119 Prorenin + H2O recombinant murine prorenin 2 Mus musculus Renin + ?
-
 ?
3.4.21.119 pyroglutamyl-Arg-Tyr-Lys-Arg-4-methyl-coumaryl-7-amide + H2O
-
 Mus musculus pyroglutamyl-Arg-Tyr-Lys-Arg + 7-amino-4-methylcoumarin
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.21.B40 EGF-binding protein
-
 Mus musculus
3.4.21.B40 K9
-
 Mus musculus
3.4.21.119 prorenin-converting enzyme
-
 Mus musculus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.21.B40 37
-
 assay at Mus musculus
3.4.21.119 37
-
 assay at Mus musculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.21.B40 7.5
-
 assay at Mus musculus
3.4.21.119 7.5
-
 assay at Mus musculus