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Literature summary extracted from

  • Yousef, G.M.; Kapadia, C.; Polymeris, M.E.; Borgono, C.; Hutchinson, S.; Wasney, G.A.; Soosaipillai, A.; Diamandis, E.P.
    The human kallikrein protein 5 (hK5) is enzymatically active, glycosylated and forms complexes with two protease inhibitors in ovarian cancer fluids (2003), Biochim. Biophys. Acta, 1628, 88-96.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.4.21.B39 medicine regulation by protease inhibitors may have potential clinical applications Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.21.B39 DNA sequence determination and analysis, expression in CHO-CCL-61 cells, in Escherichia coli, and in Pichia pastoris Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.B39 alpha1-antitrypsin
-
 Homo sapiens
3.4.21.B39 alpha2-Macroglobulin
-
 Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.B39 28000
-
 recombinant deglycosylated enzyme, gel filtration Homo sapiens
3.4.21.B39 30000
-
 recombinant glycosylated protein, gel filtration Homo sapiens
3.4.21.B39 40000
-
 native glycosylated enzyme, gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.21.B39 additional information Homo sapiens the enzyme is differentially expressed at the mRNA and protein level in diverse malignancies including cancer ?
-
 ?
3.4.21.B39 polypeptide + H2O Homo sapiens enzyme is regulated by binding to protease inhibitors peptides
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.B39 Homo sapiens Q9Y337 gene KLK5
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.21.B39 glycoprotein N-glycosylated Homo sapiens
3.4.21.B39 glycoprotein 4 potential N-glycosylation sites at amino acid residues positions 69, 173, 208, and 252 Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.B39 recombinant enzyme from CHO-CCL-61 cells and from Pichia pastoris Homo sapiens

Reaction

EC Number Reaction Comment Organism Reaction ID
3.4.21.B39 proteolytic cleavage of polypeptides enzyme is a serine protease Homo sapiens
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.21.B39 ascites fluid from ovarian cancer patients Homo sapiens
-
3.4.21.B39 milk
-
 Homo sapiens
-
3.4.21.B39 serum female and male Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.B39 FSR-amido-4-methylcoumarin + H2O fluorogenic trypsin-like peptide substrate Homo sapiens FSR + 7-amino-4-methylcoumarin
-
 ?
3.4.21.B39 additional information no activity with AAPF-amido-4-methylcoumarin Homo sapiens ?
-
 ?
3.4.21.B39 additional information enzyme shows trypsin-like but not chymotrypsin-like activity Homo sapiens ?
-
 ?
3.4.21.B39 additional information the enzyme is differentially expressed at the mRNA and protein level in diverse malignancies including cancer Homo sapiens ?
-
 ?
3.4.21.B39 polypeptide + H2O
-
 Homo sapiens peptides
-
 ?
3.4.21.B39 polypeptide + H2O enzyme is regulated by binding to protease inhibitors Homo sapiens peptides
-
 ?
3.4.21.B39 VPR-amido-4-methylcoumarin + H2O fluorogenic trypsin-like peptide substrate Homo sapiens VPR + 7-amino-4-methylcoumarin
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.21.B39 K5
-
 Homo sapiens
3.4.21.B39 kallikrein protein 5
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.21.B39 37
-
-
 Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.21.B39 8
-
 assay at Homo sapiens