Literature summary extracted from

Takayama, T.K.; McMullen, B.A.; Nelson, P.S.; Matsumura, M.; Fujikawa, K.
Characterization of hK4 (prostase), a prostate-specific serine protease: activation of the precursor of prostate specific antigen (pro-PSA) and single-chain urokinase-type plasminogen activator and degradation of prostatic acid phosphatase (2001), Biochemistry, 40, 15341-15348.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
3.4.21.B12	construction of a chimeric enzyme with exchange of the pro-piece of the zymogen for that of prostate-specific antigen PSA to create an activation site suceptible for trypsin-type proteases, expression in Escherichia coli strain BL21(DE3)	Homo sapiens

Inhibitors

EC Number	Inhibitors	Comment	Organism
3.4.21.B12	Aprotinin	complex formation with the self-activated recombinant, chimeric enzyme	Homo sapiens
3.4.21.B12	benzamidine	93% inhibition at 10 mM	Homo sapiens
3.4.21.B12	Nalpha-p-tosyl-L-lysine chloromethyl ketone	85% inhibition at 1 mM; i.e. TLCK	Homo sapiens
3.4.21.B12	Soybean trypsin inhibitor	99% inhibition at 1 mg/ml	Homo sapiens

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
3.4.21.B12	21000	-	x * 21000, self-activated recombinant, chimeric enzyme, SDS-PAGE	Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number	Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.
3.4.21.B12	52 kDa prostatic acid phosphatase + H2O	Homo sapiens	enzyme may be involved in the physiological clearance of prostatic acid phosphatase	33 kDa prostatic acid phosphatase + 19 kDa fragment	-	?
3.4.21.B12	polypeptide + H2O	Homo sapiens	enzyme plays a role in the physiologic processing of seminal plasma proteins , e.g. pro-PSA, as well as in the pathogenesis of prostate cancer through the activation of pro-PSA	peptides	-	?
3.4.21.B12	pro-prostate-specific antigen + H2O	Homo sapiens	i.e. PSA	activated prostate-specific antigen	-	?
3.4.21.B12	pro-prostate-specific antigen + H2O	Homo sapiens	activation of PSA	activated prostate-specific antigen	-	?

Organism

EC Number	Organism	UniProt	Comment	Textmining
3.4.21.B12	Homo sapiens	-	-	-

Posttranslational Modification

EC Number	Posttranslational Modification	Comment	Organism
3.4.21.B12	proteolytic modification	recombinant chimeric enzyme with exchange of the pro-piece of the zymogen for that of prostate-specific antigen PSA to create an activation site suceptible for trypsin-type proteases proteolytically activates itself	Homo sapiens

Purification (Commentary)

EC Number	Purification (Comment)	Organism
3.4.21.B12	recombinant chimeric enzyme after isolation from inclusion bodies and refolding	Homo sapiens

Renatured (Commentary)

EC Number	Renatured (Comment)	Organism
3.4.21.B12	isolation from inclusion bodies after recombinant expression in Escherichia coli, refolding with self-activation during the process	Homo sapiens

Source Tissue

EC Number	Source Tissue	Comment	Organism	Textmining
3.4.21.B12	prostate	specific	Homo sapiens	-

Specific Activity [micromol/min/mg]

EC Number	Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
3.4.21.B12	0.31	-	purified recombinant enzyme, substrate H-D-Val-L-Leu-L-Lys-4-nitroanilide	Homo sapiens
3.4.21.B12	0.67	-	purified recombinant enzyme, substrate N-benzoyl-L-Ile-L-Glu-Gly-L-Arg-4-nitroanilide	Homo sapiens
3.4.21.B12	1.12	-	purified recombinant enzyme, substrate H-D-Pro-L-Phe-L-Arg-4-nitroanilide	Homo sapiens
3.4.21.B12	1.42	-	purified recombinant enzyme, substrate H-D-Val-Leu-Arg-4-nitroanilide	Homo sapiens

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
3.4.21.B12	52 kDa prostatic acid phosphatase + H2O	recombinant self-activated, chimeric enzyme	Homo sapiens	33 kDa prostatic acid phosphatase + 19 kDa fragment	products contain 256 and 73 amino acid residues, respectively	?
3.4.21.B12	52 kDa prostatic acid phosphatase + H2O	primary cleavage site is the Arg73-Ser74 bond	Homo sapiens	33 kDa prostatic acid phosphatase + 19 kDa fragment	products contain 256 and 73 amino acid residues, respectively	?
3.4.21.B12	52 kDa prostatic acid phosphatase + H2O	inactivation of substrate	Homo sapiens	33 kDa prostatic acid phosphatase + 19 kDa fragment	products contain 256 and 73 amino acid residues, respectively	?
3.4.21.B12	52 kDa prostatic acid phosphatase + H2O	enzyme may be involved in the physiological clearance of prostatic acid phosphatase	Homo sapiens	33 kDa prostatic acid phosphatase + 19 kDa fragment	-	?
3.4.21.B12	benzoyl-L-Ile-L-Glu-Gly-L-Arg-4-nitroanilide + H2O	recombinant self-activated, chimeric enzyme	Homo sapiens	benzoyl-L-Ile-L-Glu-Gly-L-Arg + 4-nitroaniline	-	?
3.4.21.B12	benzoyl-L-Ile-L-Glu-Gly-L-Arg-4-nitroanilide + H2O	fluorogenic substrate, i.e. S-2222	Homo sapiens	benzoyl-L-Ile-L-Glu-Gly-L-Arg + 4-nitroaniline	-	?
3.4.21.B12	D-Pro-L-Phe-L-Arg-4-nitroanilide + H2O	recombinant self-activated, chimeric enzyme	Homo sapiens	D-Pro-L-Phe-L-Arg + 4-nitroaniline	-	?
3.4.21.B12	D-Pro-L-Phe-L-Arg-4-nitroanilide + H2O	fluorogenic substrate, i.e. S-2302	Homo sapiens	D-Pro-L-Phe-L-Arg + 4-nitroaniline	-	?
3.4.21.B12	D-Val-L-Leu-L-Lys-4-nitroanilide + H2O	recombinant self-activated, chimeric enzyme	Homo sapiens	D-Val-L-Leu-L-Lys + 4-nitroaniline	-	?
3.4.21.B12	D-Val-L-Leu-L-Lys-4-nitroanilide + H2O	fluorogenic substrate, i.e. S-2251	Homo sapiens	D-Val-L-Leu-L-Lys + 4-nitroaniline	-	?
3.4.21.B12	D-Val-Leu-Arg-4-nitroanilide + H2O	recombinant self-activated, chimeric enzyme	Homo sapiens	D-Val-Leu-Arg + 4-nitroaniline	-	?
3.4.21.B12	D-Val-Leu-Arg-4-nitroanilide + H2O	fluorogenic substrate, i.e. S-2266	Homo sapiens	D-Val-Leu-Arg + 4-nitroaniline	-	?
3.4.21.B12	additional information	no activity with serum albumin from human seminal plasma	Homo sapiens	?	-	?
3.4.21.B12	additional information	enzyme has a trypsin-type substrate specificty	Homo sapiens	?	-	?
3.4.21.B12	additional information	no activity with pyroglu-Gly-Arg-4-nitroanilide, i.e. S-2444, and with 3-carbomethoxypropionyl-L-Arg-L-Pro-L-Tyr-4-nitroanilide, i.e. S-2586	Homo sapiens	?	-	?
3.4.21.B12	polypeptide + H2O	-	Homo sapiens	peptides	-	?
3.4.21.B12	polypeptide + H2O	enzyme plays a role in the physiologic processing of seminal plasma proteins , e.g. pro-PSA, as well as in the pathogenesis of prostate cancer through the activation of pro-PSA	Homo sapiens	peptides	-	?
3.4.21.B12	pro-prostate-specific antigen + H2O	activation of substrate	Homo sapiens	activated prostate-specific antigen	-	?
3.4.21.B12	pro-prostate-specific antigen + H2O	recombinant self-activated, chimeric enzyme	Homo sapiens	activated prostate-specific antigen	-	?
3.4.21.B12	pro-prostate-specific antigen + H2O	i.e. PSA	Homo sapiens	activated prostate-specific antigen	-	?
3.4.21.B12	pro-prostate-specific antigen + H2O	activation of PSA	Homo sapiens	activated prostate-specific antigen	-	?
3.4.21.B12	pro-urokinase-type plasminogen activator + H2O	activation of substrate	Homo sapiens	activated urokinase-type plasminogen activator	-	?
3.4.21.B12	pro-urokinase-type plasminogen activator + H2O	recombinant self-activated, chimeric enzyme	Homo sapiens	activated urokinase-type plasminogen activator	-	?
3.4.21.B12	pro-urokinase-type plasminogen activator + H2O	i.e. pro-uPA	Homo sapiens	activated urokinase-type plasminogen activator	-	?

Subunits

EC Number	Subunits	Comment	Organism
3.4.21.B12	?	x * 21000, self-activated recombinant, chimeric enzyme, SDS-PAGE	Homo sapiens

Synonyms

EC Number	Synonyms	Comment	Organism
3.4.21.B12	K4	-	Homo sapiens

Temperature Optimum [°C]

EC Number	Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
3.4.21.B12	37	-	assay at	Homo sapiens

pH Optimum

EC Number	pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
3.4.21.B12	7.5	-	-	Homo sapiens

pH Range

EC Number	pH Minimum	pH Maximum	Comment	Organism
3.4.21.B12	5	10.3	7% of maximal activity at ph 5.0, 32% at pH 5.5, and 40% at pH 10.3	Homo sapiens