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Literature summary extracted from

  • Simmer, J.P.; Fukae, M.; Tanabe, T.; Yamakoshi, Y.; Uchida, T.; Xue, J.; Margolis, H.C.; Shimizu, M.; DeHart, B.C.; Hu, J.C.C.; Bartlett, J.D.
    Purification, characterization, and cloning of enamel matrix serine proteinase 1 (1998), J. Dent. Res., 77, 377-386.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.21.B12 DNA and amino acid sequence determination and analysis Sus scrofa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.21.B12 cytosol
-
 Sus scrofa 5829
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.21.B12 polypeptide + H2O Sus scrofa
-
 peptides
-
 ?
3.4.21.B12 polypeptide + H2O Sus scrofa EMSP1
-
 peptides
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.B12 Sus scrofa Q9XSN6 EMSP1
-
3.4.21.B12 Sus scrofa EMSP1 Q9XSN6 EMSP1
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.21.B12 glycoprotein 3 potential N-linked glycosylation sites: N134, N169, and N214 of the nucleotide sequence Sus scrofa

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.B12
-
 Sus scrofa

Reaction

EC Number Reaction Comment Organism Reaction ID
3.4.21.B12 proteolysis of polypeptides active site residues: H71, D116, and S207 Sus scrofa
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.21.B12 dental pulp
-
 Sus scrofa
-
3.4.21.B12 enamel transition-stage developing matrix Sus scrofa
-
3.4.21.B12 epithelium of enamel organ Sus scrofa
-
3.4.21.B12 additional information enzyme expression is restricted to developimg teeth Sus scrofa
-
3.4.21.B12 tooth bud unerupted, incisor Sus scrofa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.B12 amelogenin + H2O 20 kDa substrate Sus scrofa amelogenin fragments
-
 ?
3.4.21.B12 amelogenin + H2O 20 kDa substrate Sus scrofa EMSP1 amelogenin fragments
-
 ?
3.4.21.B12 Gelatin + H2O zymograph assay Sus scrofa ?
-
 ?
3.4.21.B12 Gelatin + H2O zymograph assay Sus scrofa EMSP1 ?
-
 ?
3.4.21.B12 polypeptide + H2O
-
 Sus scrofa peptides
-
 ?
3.4.21.B12 polypeptide + H2O
-
 Sus scrofa EMSP1 peptides
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.21.B12 ? x * 24100-34000, SDS-PAGE and amino acid sequence determination Sus scrofa

Synonyms

EC Number Synonyms Comment Organism
3.4.21.B12 EMSP1
-
 Sus scrofa
3.4.21.B12 serine protease 1
-
 Sus scrofa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.21.B12 37
-
 assay at Sus scrofa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.21.B12 6
-
 assay at, substrate 20 kDa amelogenin Sus scrofa
3.4.21.B12 7.5
-
 assay at, substrate gelatin Sus scrofa

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.4.21.B12 Sus scrofa
-
-
 6