EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.4.21.117 | Soybean trypsin inhibitor | strong inhibition | Rattus norvegicus | |
3.4.21.118 | additional information | no inhibition by soybean trypsin inhibitor | Rattus norvegicus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.4.21.35 | 38000 | - |
x * 38000, kallikrein k1, SDS-PAGE | Rattus norvegicus |
3.4.21.117 | 9000 | - |
x * 9000 + x * 19000, SDS-PAGE | Rattus norvegicus |
3.4.21.117 | 19000 | - |
x * 9000 + x * 19000, SDS-PAGE | Rattus norvegicus |
3.4.21.118 | 10000 | - |
x * 10000 + x * 18000, SDS-PAGE | Rattus norvegicus |
3.4.21.118 | 18000 | - |
x * 10000 + x * 18000, SDS-PAGE | Rattus norvegicus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.21.35 | polypeptide + H2O | Rattus norvegicus | - |
peptides | - |
? | |
3.4.21.117 | polypeptide + H2O | Rattus norvegicus | - |
peptides | - |
? | |
3.4.21.118 | polypeptide + H2O | Rattus norvegicus | - |
peptides | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.21.35 | Rattus norvegicus | - |
- |
- |
3.4.21.117 | Rattus norvegicus | - |
- |
- |
3.4.21.118 | Rattus norvegicus | - |
gene KLK8 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.4.21.35 | - |
Rattus norvegicus |
3.4.21.117 | - |
Rattus norvegicus |
3.4.21.118 | - |
Rattus norvegicus |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
3.4.21.117 | cleavage of proteins with aromatic side chains in the P1 position | enzyme exhibits a marked preference for cleavage downstream of Arg residues | Rattus norvegicus | |
3.4.21.118 | cleavage of amide substrates following the basic amino acids Arg or Lys at the P1 position, with a preference for Arg over Lys | kallikrein k8 exhibit a marked preference for cleavage downstream of Arg residues | Rattus norvegicus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.4.21.35 | submaxillary gland | - |
Rattus norvegicus | - |
3.4.21.117 | submandibular gland | - |
Rattus norvegicus | - |
3.4.21.118 | submaxillary gland | - |
Rattus norvegicus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.4.21.117 | additional information | - |
- |
Rattus norvegicus |
3.4.21.118 | additional information | - |
- |
Rattus norvegicus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.21.35 | benzoyl-Phe-Val-Arg-7-amido-4-methylcoumarin + H2O | - |
Rattus norvegicus | benzoyl-Phe-Val-Arg + 7-amino-4-methylcoumarin | - |
? | |
3.4.21.35 | benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O | - |
Rattus norvegicus | benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin | - |
? | |
3.4.21.35 | polypeptide + H2O | - |
Rattus norvegicus | peptides | - |
? | |
3.4.21.117 | benzoyl-Phe-Val-Arg-7-amido-4-methylcoumarin + H2O | - |
Rattus norvegicus | benzoyl-Phe-Val-Arg + 7-amino-4-methylcoumarin | - |
? | |
3.4.21.117 | benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O | - |
Rattus norvegicus | benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin | - |
? | |
3.4.21.117 | additional information | no kininogenase activity | Rattus norvegicus | ? | - |
? | |
3.4.21.117 | additional information | enzyme exhibits a marked preference for cleavage downstream of Arg residues | Rattus norvegicus | ? | - |
? | |
3.4.21.117 | o-aminobenzoyl-FRAPR-(2,4-dinitrophenyl)ethylenediamine + H2O | low activity | Rattus norvegicus | ? | - |
? | |
3.4.21.117 | o-aminobenzoyl-FRLVR-(2,4-dinitrophenyl)ethylenediamine + H2O | low activity | Rattus norvegicus | o-aminobenzoyl-FR + LVR-(2,4-dinitrophenyl)ethylenediamine | - |
? | |
3.4.21.117 | o-aminobenzoyl-SVIRRVQ-(2,4-dinitrophenyl)ethylenediamine + H2O | - |
Rattus norvegicus | ? | - |
? | |
3.4.21.117 | o-aminobenzoyl-TSVIRRPQ-(2,4-dinitrophenyl)ethylenediamine + H2O | low activity | Rattus norvegicus | ? | - |
? | |
3.4.21.117 | PFR-7-amido-4-methylcoumarin + H2O | best substrate | Rattus norvegicus | ? | - |
? | |
3.4.21.117 | polypeptide + H2O | - |
Rattus norvegicus | peptides | - |
? | |
3.4.21.117 | tert-butyloxycarbonyl-Leu-Gly-Arg-7-amido-4-methylcoumarin + H2O | - |
Rattus norvegicus | tert-butyloxycarbonyl-Leu-Gly-Arg + 7-amino-4-methylcoumarin | - |
? | |
3.4.21.117 | tert-butyloxycarbonyl-Phe-Ser-Arg-7-amido-4-methylcoumarin + H2O | - |
Rattus norvegicus | tert-butyloxycarbonyl-Phe-Ser-Arg + 7-amino-4-methylcoumarin | - |
? | |
3.4.21.118 | benzoyl-Phe-Val-Arg-4-methylcoumarin 7-amide + H2O | - |
Rattus norvegicus | benzoyl-Phe-Val-Arg + 7-amino-4-methylcoumarin | - |
? | |
3.4.21.118 | benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide + H2O | - |
Rattus norvegicus | benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin | - |
? | |
3.4.21.118 | additional information | kallikrein k8 exhibits a marked preference for cleavage downstream of Arg residues | Rattus norvegicus | ? | - |
? | |
3.4.21.118 | additional information | no activity with tert-butyloxycarbonyl-Leu-Gly-Arg-4-methylcoumarin 7-amide | Rattus norvegicus | ? | - |
? | |
3.4.21.118 | polypeptide + H2O | - |
Rattus norvegicus | peptides | - |
? | |
3.4.21.118 | Pro-Phe-Arg-4-methylcoumaryl-7-amide | - |
Rattus norvegicus | Pro-Phe-Arg + 7-amino-4-methylcoumarin | - |
? | |
3.4.21.118 | tert-butyloxycarbonyl-Phe-Ser-Arg-4-methylcoumaryl-7-amide + H2O | no activity | Rattus norvegicus | tert-butyloxycarbonyl-Phe-Ser-Arg + 7-amino-4-methylcoumarin | - |
? | |
3.4.21.118 | tert-butyloxycarbonyl-Val-Pro-Arg-4-methylcoumaryl-7-amide + H2O | - |
Rattus norvegicus | tert-butyloxycarbonyl-Val-Pro-Arg + 7-amino-4-methylcoumarin | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.4.21.35 | ? | x * 38000, kallikrein k1, SDS-PAGE | Rattus norvegicus |
3.4.21.117 | ? | x * 9000 + x * 19000, SDS-PAGE | Rattus norvegicus |
3.4.21.118 | ? | x * 10000 + x * 18000, SDS-PAGE | Rattus norvegicus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.4.21.117 | tissue kallikrein | - |
Rattus norvegicus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.4.21.117 | 37 | - |
assay at | Rattus norvegicus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.4.21.117 | 9 | - |
assay at | Rattus norvegicus |