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Literature summary extracted from

  • Kishi, T.; Kato, M.; Shimizu, T.; Kato, K.; Matsumoto, K.; Yoshida, S.; Shiosaka, S.; Hakoshima, T.
    Crystallization and preliminary X-ray analysis of neuropsin, a serine protease expressed in the limbic system of mouse brain (1997), J. Struct. Biol., 118, 248-251.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.4.21.118 pharmacology enzyme is a drug target in the treatment of epilepsy Mus musculus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.21.118 overexpression of proneuropsin in Spodoptera frugiperda insect cells via baculovirus infection Mus musculus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.4.21.118 hanging drop vapor diffusion method, recombinant enzyme, protein solution: 24.5 mg/ml, 5 mM HEPES, pH 7.4, 100 mM NaCl, 4°C, precipitant with PEG, X-ray diffraction structure determination and analysis Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.21.118 polypeptide + H2O Mus musculus enzyme is implicated in various neurological processes including formation of memory peptides
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.118 Mus musculus Q61955
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.118 recombinant from SF9 insect cells Mus musculus

Reaction

EC Number Reaction Comment Organism Reaction ID
3.4.21.118 cleavage of amide substrates following the basic amino acids Arg or Lys at the P1 position, with a preference for Arg over Lys enzyme is a trypsin-type serine protease Mus musculus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.21.118 brain
-
 Mus musculus
-
3.4.21.118 hippocampus
-
 Mus musculus
-
3.4.21.118 limbic system
-
 Mus musculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.118 polypeptide + H2O
-
 Mus musculus peptides
-
 ?
3.4.21.118 polypeptide + H2O enzyme is implicated in various neurological processes including formation of memory Mus musculus peptides
-
 ?