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Literature summary extracted from
- Human jejunal brush border folate conjugase. Characteristics and inhibition by salicylazosulfapyridine (1981), Biochim. Biophys. Acta, 659, 62-69.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.19.9 | p-hydroxymercuribenzoate | slightly enhances activity | Homo sapiens |  |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.19.9 | additional information | no inhibition by diphenylhydantoin or salicylazosulfapyridine; no inhibition by ethanol | Homo sapiens | |
| 3.4.19.9 | Salicylazosulfapyridine | - |
Homo sapiens | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.19.9 | 0.0016 | - |
Pteroyltriglutamate | - |
Homo sapiens | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.19.9 | pteroylpolyglutamate + H2O | Homo sapiens | the enzyme progressively hydrolyzes glutamyl units from pteroylpolyglutamate, leaving pteroylmonoglutamate as the folate form available for intestinal transport | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.19.9 | Homo sapiens | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.19.9 | - |
Homo sapiens |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.4.19.9 | bile | - |
Homo sapiens | - |
| 3.4.19.9 | jejunum | mucosal brush-border | Homo sapiens | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.4.19.9 | additional information | - |
- |
Homo sapiens |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.4.19.9 | -70°C, partially purified enzyme is stable for 3 months | Homo sapiens |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.19.9 | pteroyldiglutamate + H2O | - |
Homo sapiens | ? | - |
? | |
| 3.4.19.9 | pteroylglutamylhexaglutamate + H2O | - |
Homo sapiens | short-chain pteroylglutamates | after 60 min incubation, pteroylglutamate is the major product after 120 min, with quantitative recovery of free glutamate, enzyme is an exopeptidase which progressively hydrolyzes glutamyl units from pteroylpolyglutamate, leaving pteroylmonoglutamate as the folate form available for intestinal transport | ? | |
| 3.4.19.9 | pteroylhexaglutamate + H2O | - |
Homo sapiens | ? | - |
? | |
| 3.4.19.9 | pteroylpolyglutamate + H2O | the enzyme progressively hydrolyzes glutamyl units from pteroylpolyglutamate, leaving pteroylmonoglutamate as the folate form available for intestinal transport | Homo sapiens | ? | - |
? | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.19.9 | 6 | 6.5 | - |
Homo sapiens |
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