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Literature summary extracted from

  • Haley, E.E.
    beta-Aspartyl peptidase from rat liver (1970), Methods Enzymol., 19, 737-741.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.19.5 2-mercaptoethanol slight activation Rattus norvegicus

General Stability

EC Number General Stability Organism
3.4.19.5 stability is dependent on the presence of sodium ions Rattus norvegicus
3.4.19.5 stable to freezing and thawing in 0.01 M sodium phosphate buffer Rattus norvegicus
3.4.19.5 unstable to dialysis against water or 0.01 M Tris-HCl buffer, pH 8.0, if 0.01 M NaCl is included in the dialysis solution, 80% of the activity is recovered Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.19.5 p-hydroxymercuribenzoate
-
 Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
3.4.19.5 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.19.5
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.19.5 brain
-
 Rattus norvegicus
-
3.4.19.5 cardiac muscle
-
 Rattus norvegicus
-
3.4.19.5 kidney
-
 Rattus norvegicus
-
3.4.19.5 liver
-
 Rattus norvegicus
-
3.4.19.5 lung
-
 Rattus norvegicus
-
3.4.19.5 skeletal muscle
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.19.5 0.007
-
-
 Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
3.4.19.5 room temperature, stable for 5 days Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.19.5 Asp-Gly-Ala + H2O 55% of the activity with beta-aspartylglycine Rattus norvegicus Asp + Gly-Ala
-
 ?
3.4.19.5 Asp-Gly-Val + H2O 13% of the activity with beta-aspartylglycine Rattus norvegicus Asp + Gly-Val
-
 ?
3.4.19.5 beta-Asp-Ala + H2O 51% of the activity with beta-aspartylglycine Rattus norvegicus Asp + Ala
-
 ?
3.4.19.5 beta-Asp-Gly + H2O
-
 Rattus norvegicus Asp + Gly
-
 ?
3.4.19.5 beta-Asp-Gly-Gly + H2O 95% of the activity with beta-aspartylglycine Rattus norvegicus Asp + Gly-Gly
-
 ?
3.4.19.5 beta-Asp-Ile + H2O 37% of the activity with beta-aspartylglycine Rattus norvegicus Asp + Ile
-
 ?
3.4.19.5 beta-Asp-Leu + H2O 59-65% of the activity with beta-aspartylglycine Rattus norvegicus Asp + Leu
-
 ?
3.4.19.5 beta-Asp-Met + H2O 82% of the activity with beta-aspartylglycine Rattus norvegicus Asp + Met
-
 ?
3.4.19.5 beta-Asp-Ser + H2O 47-56% of the activity with beta-aspartylglycine Rattus norvegicus Asp + Ser
-
 ?
3.4.19.5 beta-Asp-Thr + H2O 29% of the activity with beta-aspartylglycine Rattus norvegicus Asp + Thr
-
 ?
3.4.19.5 beta-Asp-Val + H2O 28% of the activity with beta-aspartylglycine Rattus norvegicus Asp + Val
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.19.5 7 9 Tris-HCl buffer Rattus norvegicus
3.4.19.5 7.5 8 sodium phosphate buffer Rattus norvegicus