EC Number | General Stability | Organism |
---|---|---|
3.4.17.11 | bovine albumin stabilizes against heat inactivation | Flavobacterium sp. |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.4.17.11 | 2-mercaptoethanol | - |
Flavobacterium sp. | |
3.4.17.11 | citrate | - |
Flavobacterium sp. | |
3.4.17.11 | CoCl2 | - |
Flavobacterium sp. | |
3.4.17.11 | DTT | - |
Flavobacterium sp. | |
3.4.17.11 | EDTA | Ca2+, Co2+, Mg2+ and Zn2+ reverse inhibition | Flavobacterium sp. | |
3.4.17.11 | L-Glutamic acid | weak | Flavobacterium sp. | |
3.4.17.11 | potassium ascorbate | 1 mM: harmless, 3 mM: strong inhibition | Flavobacterium sp. | |
3.4.17.11 | Pteroic acid | - |
Flavobacterium sp. | |
3.4.17.11 | ZnCl2 | - |
Flavobacterium sp. |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.4.17.11 | Ca2+ | weak activator in absence of EDTA | Flavobacterium sp. | |
3.4.17.11 | Zn2+ | weak activator in absence of EDTA | Flavobacterium sp. |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.4.17.11 | 53000 | - |
gel filtration | Flavobacterium sp. |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.17.11 | Flavobacterium sp. | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.4.17.11 | - |
Flavobacterium sp. |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.4.17.11 | 39.43 | - |
- |
Flavobacterium sp. |
EC Number | Storage Stability | Organism |
---|---|---|
3.4.17.11 | -20°C, stable for 1 year | Flavobacterium sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.17.11 | 2,4-dihydroxypteroyl-L-glutamic acid + H2O | - |
Flavobacterium sp. | 2,4-dihydroxypteroate + L-Glu | - |
? | |
3.4.17.11 | 4-aminopteroylglutamic acid + H2O | i.e. aminopterin | Flavobacterium sp. | 4-aminopteroate + Glu | - |
? | |
3.4.17.11 | 5-formyltetrahydrofolic acid + H2O | i.e. leucovorin | Flavobacterium sp. | 5-formylpteroate + Glu | - |
? | |
3.4.17.11 | 5-methyltetrahydrofolate + H2O | weak | Flavobacterium sp. | 5-methylpteroate + Glu | - |
? | |
3.4.17.11 | folic acid + H2O | - |
Flavobacterium sp. | pteroic acid + glutamic acid | - |
? | |
3.4.17.11 | methotrexate + H2O | - |
Flavobacterium sp. | 4-amino-4-deoxy-N10-methylpteroic acid + L-glutamate | - |
? | |
3.4.17.11 | additional information | releases the carboxy-terminal glutamyl moiety of derivatives of pteroyl-mono-L-glutamic acid | Flavobacterium sp. | ? | - |
? | |
3.4.17.11 | additional information | substituents on N5 of the pteridine ring decrease the velocity of hydrolysis | Flavobacterium sp. | ? | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.4.17.11 | 30 | - |
with methotrexate and 5-methyltetrahydrofolate as substrates, the reaction proceeds equally well between 30°C and 42°C | Flavobacterium sp. |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.4.17.11 | 4 | - |
half-life: 4 h | Flavobacterium sp. |
3.4.17.11 | 25 | - |
half-life: 90 min | Flavobacterium sp. |
3.4.17.11 | 37 | - |
half-life: 45 min | Flavobacterium sp. |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.4.17.11 | 6 | 8 | hydrolysis of 5-methyltetrahydrofolate, phosphate buffer | Flavobacterium sp. |
3.4.17.11 | 6.5 | 6.8 | hydrolysis of leucoverin, phosphate buffer | Flavobacterium sp. |
3.4.17.11 | 7 | 7.5 | - |
Flavobacterium sp. |
3.4.17.11 | 7 | 8 | hydrolysis of 5-methyltetrahydrofolate, Tris buffer | Flavobacterium sp. |
3.4.17.11 | 7.2 | - |
hydrolysis of methotrexate, folic acid | Flavobacterium sp. |
3.4.17.11 | 7.5 | 8 | hydrolysis of leucoverin, Tris buffer | Flavobacterium sp. |