EC Number | Cloned (Comment) | Organism |
---|---|---|
3.4.16.6 | individual domains of the enzyme are expressed in insect Sf9 cells using the baculovirus expression system. Medium from domain 1B-expressing cells and domain 2-expressing cells shows substantial enzymatic activity, whereas medium from domain 1A-expressing cells is not different from cells infected with wild-type virus. The individual domains 1A, 1B, and 2 are expressed in baculovirus under the polyhedrin promoter and with the signal peptide of the rat enzyme | Drosophila melanogaster |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.4.16.6 | E353Q | cells infected with the mutant enzyme show considerable carboxypeptidase activity, mutation eliminates the activity of domain 1 | Drosophila melanogaster |
3.4.16.6 | E771Q | cells infected with the mutant enzyme show considerable carboxypeptidase activity, mutation eliminates the activity of domain 2 | Drosophila melanogaster |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.4.16.6 | 4-[[(3,4-dinitrophenyl)carbonyl]amino]-2-(6-hydroxy-3-oxo-3H-xanthen-9-yl)benzoic acid | - |
Drosophila melanogaster | |
3.4.16.6 | EDTA | - |
Drosophila melanogaster | |
3.4.16.6 | Guanidinoethylmercaptosuccinic acid | domain 1B and domain | Drosophila melanogaster | |
3.4.16.6 | PCMB | domain 1B and domain | Drosophila melanogaster |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.16.6 | 0.208 | - |
dansyl-Phe-Ala-Arg | pH 7.4, 37°C, domain 1B | Drosophila melanogaster | |
3.4.16.6 | 0.246 | - |
dansyl-Phe-Ala-Arg | pH 5.7, 37°C, domain 2 | Drosophila melanogaster |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.16.6 | additional information | Drosophila melanogaster | the enzyme functions in processing of proteins that transit the secretory pathway | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.16.6 | Drosophila melanogaster | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.4.16.6 | domain 1B and 2 expressed in Sf9 cells using baculovirus expression system | Drosophila melanogaster |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.16.6 | Dansyl-Phe-Ala-Arg + H2O | - |
Drosophila melanogaster | Dansyl-Phe-Ala + Arg | - |
? | |
3.4.16.6 | additional information | activity of individual domains of the enzyme. Domain 1B is more active at neutral pH and greatly prefers C-terminal Arg over Lys, whereas domain 2 is more active at pH 5-6 and slightly prefers C-terminal Lys over Arg | Drosophila melanogaster | ? | - |
? | |
3.4.16.6 | additional information | the enzyme functions in processing of proteins that transit the secretory pathway | Drosophila melanogaster | ? | - |
? |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.16.6 | 3.86 | - |
dansyl-Phe-Ala-Arg | pH 5.7, 37°C, domain 2 | Drosophila melanogaster | |
3.4.16.6 | 32.6 | - |
dansyl-Phe-Ala-Arg | pH 7.4, 37°C, domain 1B | Drosophila melanogaster |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.4.16.6 | 5 | 7 | wild-type enzyme | Drosophila melanogaster |
3.4.16.6 | 5.6 | - |
domain 1B | Drosophila melanogaster |
3.4.16.6 | 7.4 | - |
domain 2 | Drosophila melanogaster |