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Literature summary extracted from

  • Spr÷ssler, B.; Heilmann, H.D.; Grampp, E.; Uhlig, H.
    Properties of carboxypeptidase C isolated from orange leaves (1971), Hoppe-Seyler's Z. Physiol. Chem., 352, 1524-1530.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.4.16.5 citrus peel enzyme is inactivated upon lyophilization Citrus sp.
3.4.16.5 orange leaf enzyme is stable to lyophilization Citrus sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.16.5 2-propanol above 5% Citrus sp.
3.4.16.5 diisopropyl fluorophosphate peel enzyme inhibited, leaf enzyme not Citrus sp.
3.4.16.5 ethanol above 5% Citrus sp.
3.4.16.5 additional information not inhibition by EDTA Citrus sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.16.5 additional information no activation by metal ions Citrus sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.16.5 126000
-
 enzyme from peel, gel filtration Citrus sp.
3.4.16.5 175000
-
 enzyme from leaf, gel filtration Citrus sp.

Organism

EC Number Organism UniProt Comment Textmining
3.4.16.5 Citrus sp.
-
-
-
3.4.16.5 Citrus sp.
-
 orange
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.16.5
-
 Citrus sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.16.5 leaf
-
 Citrus sp.
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.16.5 benzyloxycarbonyl-Leu-Phe + H2O
-
 Citrus sp. benzyloxycarbonyl-Leu + Phe
-
 ?
3.4.16.5 additional information
-
 Citrus sp. ?
-
 ?
3.4.16.5 additional information removes C-terminal aromatic, neutral acid and basic amino acids, proline bonds are hydrolyzed on both the amino and carboxyl side, glycine is attacked slowly Citrus sp. ?
-
 ?
3.4.16.5 additional information owing to its low specificity very suitable for sequence analysis Citrus sp. ?
-
 ?
3.4.16.5 additional information no hydrolysis of benzyloxycarbonyl-Gly-Pro Citrus sp. ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.16.5 40
-
 20 min incubation, benzyloxycarbonyl-Leu-Phe Citrus sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.4.16.5 30
-
 30°C: about 65% of activity maximum, 60°C: about 70% of activity maximum Citrus sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.16.5 5.3
-
-
 Citrus sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.16.5 4.5 6 pH 4.5: about 70% of activity maximum, pH 6: about 55% of activity maximum Citrus sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.16.5 4 6 orange leaf enzyme is unstable below pH 4 and above pH 6 Citrus sp.
3.4.16.5 5
-
 most stable at room temperature Citrus sp.