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Literature summary extracted from
- Purification and characterisation of a tripeptidyl aminopeptidase I from rat spleen (1998), Biochim. Biophys. Acta, 1384, 233-242.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.14.9 | Ala-Ala-Ala-CH2Cl | competitive | Rattus norvegicus |  |
| 3.4.14.9 | Ala-Ala-Phe-CH2Cl | 0.001 mM, complete inhibition, competitive | Rattus norvegicus | |
| 3.4.14.9 | Ala-Ala-Tyr(benzoyl)-CH2Cl | competitive | Rattus norvegicus | |
| 3.4.14.9 | Ala-Ala-Tyr-CH2Cl | competitive | Rattus norvegicus | |
| 3.4.14.9 | Ala-Phe-Ala-CH2Cl | competitive | Rattus norvegicus | |
| 3.4.14.9 | bestatin | 0.01 mM, 11% inhibition | Rattus norvegicus | |
| 3.4.14.9 | diethyl dicarbonate | 61% inhibition with 1 mM, 91% inhibition with 10 mM | Rattus norvegicus | |
| 3.4.14.9 | E-64 | 0.01 mM, 22% inhibition | Rattus norvegicus | |
| 3.4.14.9 | leupeptin | 0.001 mg/ml, 17% inhibition | Rattus norvegicus | |
| 3.4.14.9 | pepstatin A | 0.1 mg/ml, 26% inhibition | Rattus norvegicus | |
| 3.4.14.9 | Phe-Ala-Ala-CH2Cl | competitive | Rattus norvegicus | |
| 3.4.14.9 | Phe-CH2Cl | 0.1 mM, 21% inhibition | Rattus norvegicus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.14.9 | 0.73 | - |
Ala-Ala-Phe-7-amido-4-methylcoumarin | pH 4.0 | Rattus norvegicus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.14.9 | 47000 | - |
1 * 47000, SDS-PAGE | Rattus norvegicus |
| 3.4.14.9 | 64000 | - |
gel filtration | Rattus norvegicus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.14.9 | Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.14.9 | - |
Rattus norvegicus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.4.14.9 | spleen | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.4.14.9 | 12.6 | - |
- |
Rattus norvegicus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.14.9 | Ala-Ala-Ala-4-nitroanilide + H2O | - |
Rattus norvegicus | Ala-Ala-Ala + 4-nitroaniline | - |
? | |
| 3.4.14.9 | Ala-Ala-Phe-4-nitroanilide + H2O | - |
Rattus norvegicus | Ala-Ala-Phe + 4-nitroaniline | - |
? | |
| 3.4.14.9 | Ala-Ala-Phe-7-amido-4-methylcoumarin + H2O | - |
Rattus norvegicus | Ala-Ala-Phe + 7-amino-4-methylcoumarin | - |
? | |
| 3.4.14.9 | DRVYIHPF + H2O | - |
Rattus norvegicus | DRV + YIHPF | - |
? | |
| 3.4.14.9 | glucagon + H2O | - |
Rattus norvegicus | additional information | all nine tripeptides and the C-terminal pentapeptide are identified | ? | |
| 3.4.14.9 | Gly-Pro-Leu 2-naphthylamide + H2O | 25% of the activity with Gly-Pro-Met 2-naphthylamide, Ala-Ala-Phe-4-nitroanilide or Ala-Ala-Phe-7-amido-4-methylcoumarin | Rattus norvegicus | Gly-Pro-Leu + 2-naphthylamine | - |
? | |
| 3.4.14.9 | Gly-Pro-Met 2-naphthylamide + H2O | - |
Rattus norvegicus | Gly-Pro-Met + 2-naphthylamine | - |
? | |
| 3.4.14.9 | additional information | the enzymne cleaves tripeptides from synthetic substrates provided that the N-terminus is unsubstituted and the amino acid in the P1 position is not charged. The enzyme also cleaves small peptides, angiotensin II and glucagon, releasing tripeptides but does not appear to demonstrate any preference for amino acids on either side of the cleavage site. Substrates with a charged amino acid in the P1 position appear to be resistant to hydrolysis | Rattus norvegicus | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.4.14.9 | monomer | 1 * 47000, SDS-PAGE | Rattus norvegicus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.14.9 | 4 | - |
hydrolysis of Ala-Ala-Phe-7-amido-4-methylcoumarin | Rattus norvegicus |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.14.9 | 3.5 | 5 | pH 3.5: about 25% of maximal activity, pH 5.0: about 45% of maximal activity, hydrolysis of Ala-Ala-Phe-7-amido-4-methylcoumarin | Rattus norvegicus |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.14.9 | 5 | 6 | 48 h, stable | Rattus norvegicus |
| 3.4.14.9 | 7 | - |
48 h, unstable above | Rattus norvegicus |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.14.9 | 0.000063 | - |
Ala-Ala-Phe-CH2Cl | pH 4.0, 37°C | Rattus norvegicus | |
| 3.4.14.9 | 0.00072 | - |
Ala-Ala-Tyr-CH2Cl | pH 4.0, 37°C | Rattus norvegicus | |
| 3.4.14.9 | 0.00081 | - |
Ala-Ala-Tyr(benzoyl)-CH2Cl | pH 4.0, 37°C | Rattus norvegicus | |
| 3.4.14.9 | 0.0013 | - |
Phe-Ala-Ala-CH2Cl | pH 4.0, 37°C | Rattus norvegicus | |
| 3.4.14.9 | 0.0069 | - |
Ala-Ala-Ala-CH2Cl | pH 4.0, 37°C | Rattus norvegicus | |
| 3.4.14.9 | 0.0079 | - |
Ala-Phe-Ala-CH2Cl | pH 4.0, 37°C | Rattus norvegicus | |
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