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Literature summary extracted from

  • Krimper, R.P.; Jones, T.H.
    Purification and characterization of tripeptidyl peptidase I from Dictyostelium discoideum (1999), Biochem. Mol. Biol. Int., 47, 1079-1088.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.14.9 2-mercaptoethanol 0.6 mM, complete inactivation Dictyostelium discoideum
3.4.14.9 angiotensin II 0.3 mM, 22% inhibition Dictyostelium discoideum
3.4.14.9 angiotensin III 0.3 mM, 18% inhibition Dictyostelium discoideum
3.4.14.9 Aprotinin 0.08 mM, 60% loss of activity Dictyostelium discoideum
3.4.14.9 dithiothreitol 0.6 mM, complete inactivation Dictyostelium discoideum
3.4.14.9 EDTA moderate Dictyostelium discoideum
3.4.14.9 glutathione 0.6 mM, 93% loss of activity Dictyostelium discoideum
3.4.14.9 Gly-Ala-Ala 0.3 mM, 36% inhibition Dictyostelium discoideum
3.4.14.9 Gly-His-Lys 0.3 mM, 49% inhibition Dictyostelium discoideum
3.4.14.9 Leu-Ala-Pro 0.3 mM, 55% inhibition Dictyostelium discoideum
3.4.14.9 Lys-Ala-Ala 0.3 mM, 34% inhibition Dictyostelium discoideum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.14.9 0.0268
-
 Ala-Ala-Phe-4-nitroanilide pH 4.5 Dictyostelium discoideum
3.4.14.9 0.437
-
 Ala-Phe-Pro-p-nitroanilide pH 4.5 Dictyostelium discoideum
3.4.14.9 0.888
-
 Ala-Ala-Pro-4-nitroanilide pH 4.5 Dictyostelium discoideum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.14.9 107000
-
 2 * 107000, SDS-PAGE Dictyostelium discoideum
3.4.14.9 217000
-
 gel filtration Dictyostelium discoideum

Organism

EC Number Organism UniProt Comment Textmining
3.4.14.9 Dictyostelium discoideum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.14.9
-
 Dictyostelium discoideum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.14.9 171
-
-
 Dictyostelium discoideum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.14.9 Ala-Ala-Ala-4-nitroanilide + H2O
-
 Dictyostelium discoideum Ala-Ala-Ala + 4-nitroaniline
-
 ?
3.4.14.9 Ala-Ala-Phe-4-nitroanilide + H2O
-
 Dictyostelium discoideum Ala-Ala-Phe + 4-nitroaniline
-
 ?
3.4.14.9 Ala-Ala-Pro-4-nitroanilide + H2O
-
 Dictyostelium discoideum Ala-Ala-Pro + 4-nitroaniline
-
 ?
3.4.14.9 Ala-Phe-Pro-4-nitroanilide + H2O
-
 Dictyostelium discoideum Ala-Phe-Pro + 4-nitroaniline
-
 ?
3.4.14.9 additional information no significant aminopeptidase activity Dictyostelium discoideum ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.14.9 dimer 2 * 107000, SDS-PAGE Dictyostelium discoideum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.14.9 140000
-
 Ala-Phe-Pro-p-nitroanilide pH 4.5 Dictyostelium discoideum
3.4.14.9 210000
-
 Ala-Ala-Pro-4-nitroanilide pH 4.5 Dictyostelium discoideum
3.4.14.9 550000
-
 Ala-Ala-Phe-4-nitroanilide pH 4.5 Dictyostelium discoideum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.14.9 4.5
-
 and a second optimum at pH 5.9 Dictyostelium discoideum
3.4.14.9 5.9
-
 and a second optimum at pH 4.5, at pH 5.9 70% of the activity at pH 4.5 Dictyostelium discoideum