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Literature summary extracted from
- An X-prolyl dipeptidyl aminopeptidase (pepX) gene from Lactobacillus helveticus (1995), Microbiology, 141, 3067-3075.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.4.14.11 | - |
Lactobacillus helveticus |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.14.11 | 1,10-phenanthroline | not | Lactobacillus helveticus |  |
| 3.4.14.11 | Cu2+ | - |
Lactobacillus helveticus | |
| 3.4.14.11 | EDTA | no inhibition | Lactobacillus helveticus | |
| 3.4.14.11 | p-hydroxymercuribenzoate | reactivated by DTT | Lactobacillus helveticus | |
| 3.4.14.11 | phenylmethylsulfonyl fluoride | - |
Lactobacillus helveticus | |
| 3.4.14.11 | Zn2+ | - |
Lactobacillus helveticus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.14.11 | 0.28 | - |
Gly-Pro 4-nitroanilide | - |
Lactobacillus helveticus | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.14.11 | 90573 | - |
2 * 90573, calculation from nucleotide sequence | Lactobacillus helveticus |
| 3.4.14.11 | 165000 | - |
gel filtration | Lactobacillus helveticus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.14.11 | Lactobacillus helveticus | - |
- |
- |
| 3.4.14.11 | Lactobacillus helveticus 53/7 | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.14.11 | - |
Lactobacillus helveticus |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.4.14.11 | 90 | - |
- |
Lactobacillus helveticus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.14.11 | Arg-Pro 4-nitroanilide + H2O | - |
Lactobacillus helveticus | Arg-Pro + 4-nitroaniline | - |
? | |
| 3.4.14.11 | Arg-Pro 4-nitroanilide + H2O | - |
Lactobacillus helveticus 53/7 | Arg-Pro + 4-nitroaniline | - |
? | |
| 3.4.14.11 | Gly-Pro 4-nitroanilide + H2O | - |
Lactobacillus helveticus | Gly-Pro + 4-nitroaniline | - |
? | |
| 3.4.14.11 | Gly-Pro 4-nitroanilide + H2O | - |
Lactobacillus helveticus 53/7 | Gly-Pro + 4-nitroaniline | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.4.14.11 | dimer | 2 * 90573, calculation from nucleotide sequence | Lactobacillus helveticus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.14.11 | 37 | - |
- |
Lactobacillus helveticus |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.14.11 | 10 | 50 | about 55% of activity maximum at 10°C and 50°C | Lactobacillus helveticus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.14.11 | 6.5 | - |
- |
Lactobacillus helveticus |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.14.11 | 5.5 | 8 | pH 5.5: about 50% of maximal activity, pH 8: about 65% of maximal activity | Lactobacillus helveticus |
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