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Literature summary extracted from

  • Renn, S.C.; Tomkinson, B.; Taghert, P.H.
    Characterization and cloning of tripeptidyl peptidase II from the fruit fly, Drosophila melanogaster (1998), J. Biol. Chem., 273, 19173-19182.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.14.10
-
Drosophila melanogaster

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.14.10 butabindide 0.002 mM, 83% inhibition Drosophila melanogaster
3.4.14.10 butabindide
-
Rattus norvegicus
3.4.14.10 NEM 1 mM, 87% inhibition Drosophila melanogaster
3.4.14.10 PCMB 0.2 mM, 97% inhibition Drosophila melanogaster
3.4.14.10 PMSF 1 mM nearly complete inactivation Drosophila melanogaster

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.14.10 membrane cholecystokinin-8-inactivating peptidase is a membrane bound isoform Rattus norvegicus 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.14.10 150000
-
gel filtration Drosophila melanogaster

Organism

EC Number Organism UniProt Comment Textmining
3.4.14.10 Drosophila melanogaster
-
-
-
3.4.14.10 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.14.10
-
Drosophila melanogaster

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.14.10 cerebellum
-
Rattus norvegicus
-
3.4.14.10 embryo higher specific activity in extracts from embryos as compared with adult Drosophila melanogaster
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.14.10 additional information
-
-
Drosophila melanogaster

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.14.10 Ala-Ala-Ala-p-nitroanilide + H2O 24% of the activity with Ala-Ala-Phe-p-nitroanilide Drosophila melanogaster Ala-Ala-Ala + p-nitroaniline
-
?
3.4.14.10 Ala-Ala-Phe-p-nitroanilide + H2O
-
Drosophila melanogaster Ala-Ala-Phe + p-nitroaniline
-
?
3.4.14.10 Gly-Val-Leu-Arg-Arg-Ala-Ser-Val-Ala + H2O
-
Drosophila melanogaster Gly-Val-Leu + Arg-Arg-Ala-Ser-Val-Ala
-
?
3.4.14.10 Pro-Pro-Ala-p-nitroanilide + H2O 12% of the activity with Ala-Ala-Phe-p-nitroanilide Drosophila melanogaster Pro-Pro-Ala-p-nitroaniline
-
?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.14.10 7.5 7.8 reaction with Ala-Ala-Phe-p-nitroanilide Drosophila melanogaster

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.4.14.10 0.0001
-
butabindide pH 7.5, 37°C Drosophila melanogaster