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Literature summary extracted from

  • Jackson, M.C.; Kucera, C.M.; Lenney, J.F.
    Purification and properties of human serum carnosinase (1991), Clin. Chim. Acta, 196, 193-206.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.4.13.20 inactivation during DEAE-cellulose chromatography, slow reactivation when concentrated and stored at 4°C Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.13.20 75000
-
2 * 75000, subunits are connected by one or more disulfide bonds, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.13.20 anserine + H2O Homo sapiens splitting in the blood stream beta-Ala + Ntau-methyl-L-histidine
-
?
3.4.13.20 carnosine + H2O Homo sapiens splitting in the blood stream beta-Ala + His
-
?
3.4.13.20 homocarnosine + H2O Homo sapiens splitting of homocarnosine in the brain ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.4.13.20 Homo sapiens
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.13.20 glycoprotein
-
Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.13.20
-
Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.13.20 blood serum not in serum of other mammals Homo sapiens
-
3.4.13.20 brain
-
Homo sapiens
-
3.4.13.20 additional information the enzyme activity in tissues is roughly proportional to blood content for all the tissues except brain, indicating that serum carnosinase is present in the trapped blood of these tissues Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.13.20 additional information
-
-
Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.13.20 Ala-Ala + H2O 11% of the activity with carnosine Homo sapiens Ala + Ala
-
?
3.4.13.20 Ala-His + H2O 38% of the activity with carnosine Homo sapiens Ala + His
-
?
3.4.13.20 Ala-Leu + H2O 13% of the activity with carnosine Homo sapiens Ala + Leu
-
?
3.4.13.20 Ala-Tyr + H2O 8% of the activity with carnosine Homo sapiens Ala + Tyr
-
?
3.4.13.20 anserine + H2O 88% of the activity with carnosine Homo sapiens beta-Ala + Ntau-methyl-L-histidine
-
?
3.4.13.20 anserine + H2O splitting in the blood stream Homo sapiens beta-Ala + Ntau-methyl-L-histidine
-
?
3.4.13.20 beta-Ala-Ala + H2O 10% of the activity with carnosine Homo sapiens beta-Ala + Ala
-
?
3.4.13.20 beta-Ala-Phe + H2O 18% of the activity with carnosine Homo sapiens beta-Ala + Phe
-
?
3.4.13.20 carnosine + H2O beta-Ala-His Homo sapiens beta-Ala + His
-
?
3.4.13.20 carnosine + H2O splitting in the blood stream Homo sapiens beta-Ala + His
-
?
3.4.13.20 Gly-Gly + H2O 7% of the activity with carnosine Homo sapiens Gly + Gly
-
?
3.4.13.20 Gly-His + H2O 37% of the activity with carnosine Homo sapiens Gly + His
-
?
3.4.13.20 Gly-His-Gly + H2O 13% of the activity with carnosine Homo sapiens ?
-
?
3.4.13.20 Gly-His-Lys + H2O 7% of the activity with carnosine Homo sapiens ?
-
?
3.4.13.20 Gly-Leu + H2O 21% of the activity with carnosine Homo sapiens Gly + Leu
-
?
3.4.13.20 homocarnosine + H2O 11% of the activity with carnosine Homo sapiens ?
-
?
3.4.13.20 homocarnosine + H2O splitting of homocarnosine in the brain Homo sapiens ?
-
?
3.4.13.20 Leu-Leu + H2O 8% of the activity with carnosine Homo sapiens Leu + Leu
-
?
3.4.13.20 additional information dipeptide with histidine in the C-terminal position is preferred Homo sapiens ?
-
?
3.4.13.20 Phe-Ala + H2O 8% of the activity with carnosine Homo sapiens Phe + Ala
-
?
3.4.13.20 Ser-His + H2O 8% of the activity with carnosine Homo sapiens Ser + His
-
?

Subunits

EC Number Subunits Comment Organism
3.4.13.20 dimer 2 * 75000, subunits are connected by one or more disulfide bonds, SDS-PAGE Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.13.20 4.4
-
-
Homo sapiens