EC Number | Cloned (Comment) | Organism |
---|---|---|
3.4.11.18 | full-length and truncated forms of hMetAP2 | Homo sapiens |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.4.11.18 | additional information | the 107 N-terminal residues of MetAP2 are not required for either the peptidolytic activity of the enzyme or its stability. The N-terminally truncated enzyme and the full-length enzyme yield essentially identical kinetic behavior and physical properties | Homo sapiens |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.4.11.18 | 7-amino-4-methylcoumarin | product inhibition | Homo sapiens | |
3.4.11.18 | Co2+ | 1.0 mM CoCl2, 58% inhibition | Homo sapiens | |
3.4.11.18 | fumagillin | - |
Homo sapiens | |
3.4.11.18 | L-Met | product inhibition | Homo sapiens | |
3.4.11.18 | L-Met-(RS)-sulfoxide | - |
Homo sapiens | |
3.4.11.18 | L-Nle | product inhibition | Homo sapiens | |
3.4.11.18 | Mg2+ | 1.0 mM MgCl2, 21% inhibition | Homo sapiens | |
3.4.11.18 | Mn2+ | 0.1 mM MnCl2, 26% inhibition. 5.0 mM, 36% inhibition | Homo sapiens | |
3.4.11.18 | Zn2+ | 0.1 mM, complete inhibition | Homo sapiens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.11.18 | additional information | - |
additional information | Km-values of truncated enzsme | Homo sapiens | |
3.4.11.18 | 0.12 | - |
Met-7-amido-4-methylcoumarin | pH 7.5, 30°C, in presence of 0.01 mM CoCl2 | Homo sapiens | |
3.4.11.18 | 0.34 | - |
Met-Gly-Met-Met | pH 7.5, 30°C | Homo sapiens | |
3.4.11.18 | 0.38 | - |
Met-Ala-Ser | pH 7.5, 30°C | Homo sapiens | |
3.4.11.18 | 0.67 | - |
Met-4-nitroanilide | pH 7.5, 30°C | Homo sapiens | |
3.4.11.18 | 0.85 | - |
Met-Ser | pH 7.5, 30°C | Homo sapiens | |
3.4.11.18 | 1.2 | - |
Met-Val | pH 7.5, 30°C | Homo sapiens | |
3.4.11.18 | 1.3 | - |
Met-Thr | pH 7.5, 30°C | Homo sapiens | |
3.4.11.18 | 2 | - |
Nle-4-nitroanilide | pH 7.5, 30°C | Homo sapiens | |
3.4.11.18 | 2.8 | - |
Met-Pro | pH 7.5, 30°C | Homo sapiens |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.4.11.18 | Co2+ | at low concentrations, 0.01 mM, Co2+ plays a key role in the stimulation of enzyme activity. It is still questionable that Co(II) is the physically relevant divalent cation | Homo sapiens |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.4.11.18 | 52790 | - |
MALDI-MS | Homo sapiens |
3.4.11.18 | 54700 | - |
analytical ultracentrifugation | Homo sapiens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.11.18 | Met-peptide + H2O | Homo sapiens | the enzyme is directly involved in the regulation of growth factor-stimulated endothelial cell proliferation | Met + peptide | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.11.18 | Homo sapiens | - |
methionine aminopeptidase type 2 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.4.11.18 | recombinant enzyme | Homo sapiens |
EC Number | Storage Stability | Organism |
---|---|---|
3.4.11.18 | 4°C, MetAP2 is stable for several months | Homo sapiens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.11.18 | Met-4-nitroanilide + H2O | - |
Homo sapiens | Met + 4-nitroaniline | - |
? | |
3.4.11.18 | Met-7-amido-4-methylcoumarin + H2O | - |
Homo sapiens | Met + 7-amino-4-methylcoumarin | - |
? | |
3.4.11.18 | Met-Ala + H2O | - |
Homo sapiens | Met + Ala | - |
? | |
3.4.11.18 | Met-Ala-Ser + H2O | - |
Homo sapiens | Met + Ala-Ser | - |
? | |
3.4.11.18 | Met-Gly-Met-Met + H2O | - |
Homo sapiens | Gly-Met-Met + Met | - |
? | |
3.4.11.18 | Met-Gly-Met-Met + H2O | - |
Homo sapiens | Met + Gly-Met-Met | - |
? | |
3.4.11.18 | Met-peptide + H2O | the enzyme is directly involved in the regulation of growth factor-stimulated endothelial cell proliferation | Homo sapiens | Met + peptide | - |
? | |
3.4.11.18 | Met-Pro + H2O | - |
Homo sapiens | Met + Pro | - |
? | |
3.4.11.18 | Met-Ser + H2O | - |
Homo sapiens | Met + Ser | - |
? | |
3.4.11.18 | Met-Thr + H2O | - |
Homo sapiens | Met + Thr | - |
? | |
3.4.11.18 | Met-Val + H2O | - |
Homo sapiens | Met + Val | - |
? | |
3.4.11.18 | additional information | while the nature of the P1' residue of peptide substrates dictates the substrate specificity in the active site of metAP2 the P2' residue appears to play a key role in the kinetics of peptidolysis. The catalytic efficiency of dipeptide substrates is at least 250fold lower than those of the tripeptides. When the P1' amino acid of the peptide substrate is sterically large such as with L-Leu, MetAP2 exhibits no detectable activity. L-Nle is the only amino acid residue other than L-Met that can be accomodated at the N-terminus of a substrate | Homo sapiens | ? | - |
? | |
3.4.11.18 | Nle-4-nitroanilide + H2O | - |
Homo sapiens | Nle + 4-nitroaniline | - |
? |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.11.18 | additional information | - |
additional information | turnover-numbers of truncated enzyme | Homo sapiens | |
3.4.11.18 | 0.00617 | - |
Met-Ser | pH 7.5, 30°C | Homo sapiens | |
3.4.11.18 | 0.01 | - |
Met-Thr | pH 7.5, 30°C | Homo sapiens | |
3.4.11.18 | 0.0133 | - |
Met-Val | pH 7.5, 30°C | Homo sapiens | |
3.4.11.18 | 0.0633 | - |
Met-7-amido-4-methylcoumarin | pH 7.5, 30°C, in presence of 0.01 mM CoCl2 | Homo sapiens | |
3.4.11.18 | 0.0733 | - |
Nle-4-nitroanilide | pH 7.5, 30°C | Homo sapiens | |
3.4.11.18 | 0.13 | - |
Met-Pro | pH 7.5, 30°C | Homo sapiens | |
3.4.11.18 | 0.158 | - |
Met-4-nitroanilide | pH 7.5, 30°C | Homo sapiens | |
3.4.11.18 | 2.83 | - |
Met-Gly-Met-Met | pH 7.5, 30°C | Homo sapiens | |
3.4.11.18 | 3.5 | - |
Met-Ala-Ser | pH 7.5, 30°C | Homo sapiens |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.11.18 | 0.0000016 | - |
fumagillin | pH 7.5, 30°C | Homo sapiens | |
3.4.11.18 | 0.02 | - |
L-Met | pH 7.5, 30°C | Homo sapiens | |
3.4.11.18 | 0.18 | - |
L-Met-(RS)-sulfoxide | pH 7.5, 30°C | Homo sapiens | |
3.4.11.18 | 0.29 | - |
7-amino-4-methylcoumarin | pH 7.5, 30°C | Homo sapiens |