BRENDA - Enzyme Database

Aminopeptidase Y, a new aminopeptidase from Saccharomyces cerevisiae. Purification, properties, localization, and processing by protease B

Yasuhara, T.; Nakai, T.; Ohashi, A.; J. Biol. Chem. 269, 13644-13650 (1994) View publication on PubMed

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.4.11.15
additional information
activation of 75000 Da-proform to 70000 Da active form by protease B
Saccharomyces cerevisiae
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.4.11.15
1,10-phenanthroline
95% inhibition at 1 mM
Saccharomyces cerevisiae
3.4.11.15
amastatin
90% inhibition at 0.02 mM
Saccharomyces cerevisiae
3.4.11.15
bestatin
48% inhibition at 0.02 mM
Saccharomyces cerevisiae
3.4.11.15
Cu2+
90% inhibition at 0.5 mM
Saccharomyces cerevisiae
3.4.11.15
diphosphate
93% inhibition at 1 mM
Saccharomyces cerevisiae
3.4.11.15
dithiothreitol
94% inhibition at 1 mM
Saccharomyces cerevisiae
3.4.11.15
EDTA
99% inhibition at 1 mM
Saccharomyces cerevisiae
3.4.11.15
EGTA
99.9% inhibition at 1 mM
Saccharomyces cerevisiae
3.4.11.15
Mn2+
67% inhibition at 0.5 mM
Saccharomyces cerevisiae
3.4.11.15
p-chloromercuribenzoic acid
81% inhibition at 0.1 mM
Saccharomyces cerevisiae
3.4.11.15
Triphosphate
99.9% inhibition at 1 mM
Saccharomyces cerevisiae
3.4.11.15
Zn2+
0.81-0.84 mol per mol enzyme; 72% inhibition at 0.5 mM
Saccharomyces cerevisiae
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.4.11.15
autophagic vacuole
-
Saccharomyces cerevisiae
-
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.4.11.15
Co2+
required
Saccharomyces cerevisiae
3.4.11.15
Co2+
800-900% activation by 0.5 mM
Saccharomyces cerevisiae
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.4.11.15
70000
-
1 * 70000, SDS-PAGE
Saccharomyces cerevisiae
Organism
EC Number
Organism
UniProt
Commentary
Textmining
3.4.11.15
Saccharomyces cerevisiae
-
2 molecular forms
-
Posttranslational Modification
EC Number
Posttranslational Modification
Commentary
Organism
3.4.11.15
glycoprotein
-
Saccharomyces cerevisiae
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
3.4.11.15
-
Saccharomyces cerevisiae
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.4.11.15
additional information
-
substrate-dependent
Saccharomyces cerevisiae
3.4.11.15
0.028
-
-
Saccharomyces cerevisiae
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
3.4.11.15
(Arg)3 + H2O
-
647039
Saccharomyces cerevisiae
Arg + Arg-Arg
-
647039
Saccharomyces cerevisiae
ir
3.4.11.15
(Leu)3 + H2O
-
647039
Saccharomyces cerevisiae
Leu + Leu-Leu
-
647039
Saccharomyces cerevisiae
ir
3.4.11.15
(Lys)3 + H2O
-
647039
Saccharomyces cerevisiae
Lys + Lys-Lys
-
647039
Saccharomyces cerevisiae
ir
3.4.11.15
Ala-7-amido-4-methylcoumarin + H2O
-
647039
Saccharomyces cerevisiae
Ala + 7-amino-4-methylcoumarin
-
647039
Saccharomyces cerevisiae
ir
3.4.11.15
Arg-7-amido-4-methylcoumarin + H2O
-
647039
Saccharomyces cerevisiae
Arg + 7-amino-4-methylcoumarin
-
647039
Saccharomyces cerevisiae
ir
3.4.11.15
Arg-Arg-4-methylcoumaryl-7-amide + H2O
-
647039
Saccharomyces cerevisiae
Arg + Arg-4-methylcoumaryl-7-amide
-
647039
Saccharomyces cerevisiae
ir
3.4.11.15
Arg-Arg-Lys-Ala-Leu-Ser-Pro + H2O
-
647039
Saccharomyces cerevisiae
Arg + Arg-Lys-Ala-Leu-Ser-Pro
-
647039
Saccharomyces cerevisiae
ir
3.4.11.15
Arg-Gly-Tyr-Ser-Leu-Gly + H2O
-
647039
Saccharomyces cerevisiae
Arg + Gly-Tyr-Ser-Leu-Gly
-
647039
Saccharomyces cerevisiae
ir
3.4.11.15
Arg-Val + H2O
-
647039
Saccharomyces cerevisiae
Arg + Val
-
647039
Saccharomyces cerevisiae
ir
3.4.11.15
Arg-Val-OMe + H2O
-
647039
Saccharomyces cerevisiae
Arg + Val-OMe
-
647039
Saccharomyces cerevisiae
ir
3.4.11.15
Arg-Val-Tyr-Ile-His-Pro-Phe + H2O
-
647039
Saccharomyces cerevisiae
Arg + Val-Tyr-Ile-His-Pro-Phe
-
647039
Saccharomyces cerevisiae
ir
3.4.11.15
Leu-7-amido-4-methylcoumarin + H2O
-
647039
Saccharomyces cerevisiae
Leu + 7-amino-4-methylcoumarin
-
647039
Saccharomyces cerevisiae
ir
3.4.11.15
Leu-Arg-Arg-Ala-Ser-Leu-Gly + H2O
-
647039
Saccharomyces cerevisiae
Leu + Arg-Arg-Ala-Ser-Leu-Gly
-
647039
Saccharomyces cerevisiae
ir
3.4.11.15
Leu-Leu + H2O
-
647039
Saccharomyces cerevisiae
Leu + Leu
-
647039
Saccharomyces cerevisiae
ir
3.4.11.15
Leu-Leu-OMe + H2O
-
647039
Saccharomyces cerevisiae
Leu + Leu-OMe
-
647039
Saccharomyces cerevisiae
ir
3.4.11.15
Lys-7-amido-4-methylcoumarin + H2O
-
647039
Saccharomyces cerevisiae
Lys + 7-amino-4-methylcoumarin
-
647039
Saccharomyces cerevisiae
ir
3.4.11.15
Lys-Ala-4-methylcoumaryl-7-amide + H2O
-
647039
Saccharomyces cerevisiae
Lys + Ala-4-methylcoumaryl-7-amide
-
647039
Saccharomyces cerevisiae
ir
3.4.11.15
Met-7-amido-4-methylcoumarin + H2O
-
647039
Saccharomyces cerevisiae
Met + 7-amino-4-methylcoumarin
-
647039
Saccharomyces cerevisiae
ir
3.4.11.15
Met-Ala-Ser + H2O
-
647039
Saccharomyces cerevisiae
Met + Ala-Ser
-
647039
Saccharomyces cerevisiae
ir
3.4.11.15
Met-Arg-Phe + H2O
-
647039
Saccharomyces cerevisiae
Met + Arg-Phe
-
647039
Saccharomyces cerevisiae
ir
3.4.11.15
Met-Gly-Gly + H2O
-
647039
Saccharomyces cerevisiae
Met + Gly-Gly
-
647039
Saccharomyces cerevisiae
ir
3.4.11.15
additional information
generally higher activities with dipeptide-4-methylcoumaryl-7-amides than with amino-acid-4-methylcoumaryl-7-amides
647039
Saccharomyces cerevisiae
?
-
-
-
?
3.4.11.15
Pro-Phe-Arg-4-methylcoumaryl-7-amide + H2O
-
647039
Saccharomyces cerevisiae
Pro + Phe-Arg-4-methylcoumaryl-7-amide
-
647039
Saccharomyces cerevisiae
ir
3.4.11.15
Ser-7-amido-4-methylcoumarin + H2O
-
647039
Saccharomyces cerevisiae
Ser + 7-amino-4-methylcoumarin
-
647039
Saccharomyces cerevisiae
ir
3.4.11.15
Tyr-Gly-Gly-Phe-Leu + H2O
-
647039
Saccharomyces cerevisiae
Tyr + Gly-Gly-Phe-Leu
-
647039
Saccharomyces cerevisiae
ir
Subunits
EC Number
Subunits
Commentary
Organism
3.4.11.15
monomer
1 * 70000, SDS-PAGE
Saccharomyces cerevisiae
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.4.11.15
additional information
activation of 75000 Da-proform to 70000 Da active form by protease B
Saccharomyces cerevisiae
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.4.11.15
1,10-phenanthroline
95% inhibition at 1 mM
Saccharomyces cerevisiae
3.4.11.15
amastatin
90% inhibition at 0.02 mM
Saccharomyces cerevisiae
3.4.11.15
bestatin
48% inhibition at 0.02 mM
Saccharomyces cerevisiae
3.4.11.15
Cu2+
90% inhibition at 0.5 mM
Saccharomyces cerevisiae
3.4.11.15
diphosphate
93% inhibition at 1 mM
Saccharomyces cerevisiae
3.4.11.15
dithiothreitol
94% inhibition at 1 mM
Saccharomyces cerevisiae
3.4.11.15
EDTA
99% inhibition at 1 mM
Saccharomyces cerevisiae
3.4.11.15
EGTA
99.9% inhibition at 1 mM
Saccharomyces cerevisiae
3.4.11.15
Mn2+
67% inhibition at 0.5 mM
Saccharomyces cerevisiae
3.4.11.15
p-chloromercuribenzoic acid
81% inhibition at 0.1 mM
Saccharomyces cerevisiae
3.4.11.15
Triphosphate
99.9% inhibition at 1 mM
Saccharomyces cerevisiae
3.4.11.15
Zn2+
0.81-0.84 mol per mol enzyme
Saccharomyces cerevisiae
3.4.11.15
Zn2+
72% inhibition at 0.5 mM
Saccharomyces cerevisiae
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.4.11.15
autophagic vacuole
-
Saccharomyces cerevisiae
-
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.4.11.15
Co2+
required
Saccharomyces cerevisiae
3.4.11.15
Co2+
800-900% activation by 0.5 mM
Saccharomyces cerevisiae
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.4.11.15
70000
-
1 * 70000, SDS-PAGE
Saccharomyces cerevisiae
Posttranslational Modification (protein specific)
EC Number
Posttranslational Modification
Commentary
Organism
3.4.11.15
glycoprotein
-
Saccharomyces cerevisiae
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.4.11.15
-
Saccharomyces cerevisiae
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.4.11.15
additional information
-
substrate-dependent
Saccharomyces cerevisiae
3.4.11.15
0.028
-
-
Saccharomyces cerevisiae
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
3.4.11.15
(Arg)3 + H2O
-
647039
Saccharomyces cerevisiae
Arg + Arg-Arg
-
647039
Saccharomyces cerevisiae
ir
3.4.11.15
(Leu)3 + H2O
-
647039
Saccharomyces cerevisiae
Leu + Leu-Leu
-
647039
Saccharomyces cerevisiae
ir
3.4.11.15
(Lys)3 + H2O
-
647039
Saccharomyces cerevisiae
Lys + Lys-Lys
-
647039
Saccharomyces cerevisiae
ir
3.4.11.15
Ala-7-amido-4-methylcoumarin + H2O
-
647039
Saccharomyces cerevisiae
Ala + 7-amino-4-methylcoumarin
-
647039
Saccharomyces cerevisiae
ir
3.4.11.15
Arg-7-amido-4-methylcoumarin + H2O
-
647039
Saccharomyces cerevisiae
Arg + 7-amino-4-methylcoumarin
-
647039
Saccharomyces cerevisiae
ir
3.4.11.15
Arg-Arg-4-methylcoumaryl-7-amide + H2O
-
647039
Saccharomyces cerevisiae
Arg + Arg-4-methylcoumaryl-7-amide
-
647039
Saccharomyces cerevisiae
ir
3.4.11.15
Arg-Arg-Lys-Ala-Leu-Ser-Pro + H2O
-
647039
Saccharomyces cerevisiae
Arg + Arg-Lys-Ala-Leu-Ser-Pro
-
647039
Saccharomyces cerevisiae
ir
3.4.11.15
Arg-Gly-Tyr-Ser-Leu-Gly + H2O
-
647039
Saccharomyces cerevisiae
Arg + Gly-Tyr-Ser-Leu-Gly
-
647039
Saccharomyces cerevisiae
ir
3.4.11.15
Arg-Val + H2O
-
647039
Saccharomyces cerevisiae
Arg + Val
-
647039
Saccharomyces cerevisiae
ir
3.4.11.15
Arg-Val-OMe + H2O
-
647039
Saccharomyces cerevisiae
Arg + Val-OMe
-
647039
Saccharomyces cerevisiae
ir
3.4.11.15
Arg-Val-Tyr-Ile-His-Pro-Phe + H2O
-
647039
Saccharomyces cerevisiae
Arg + Val-Tyr-Ile-His-Pro-Phe
-
647039
Saccharomyces cerevisiae
ir
3.4.11.15
Leu-7-amido-4-methylcoumarin + H2O
-
647039
Saccharomyces cerevisiae
Leu + 7-amino-4-methylcoumarin
-
647039
Saccharomyces cerevisiae
ir
3.4.11.15
Leu-Arg-Arg-Ala-Ser-Leu-Gly + H2O
-
647039
Saccharomyces cerevisiae
Leu + Arg-Arg-Ala-Ser-Leu-Gly
-
647039
Saccharomyces cerevisiae
ir
3.4.11.15
Leu-Leu + H2O
-
647039
Saccharomyces cerevisiae
Leu + Leu
-
647039
Saccharomyces cerevisiae
ir
3.4.11.15
Leu-Leu-OMe + H2O
-
647039
Saccharomyces cerevisiae
Leu + Leu-OMe
-
647039
Saccharomyces cerevisiae
ir
3.4.11.15
Lys-7-amido-4-methylcoumarin + H2O
-
647039
Saccharomyces cerevisiae
Lys + 7-amino-4-methylcoumarin
-
647039
Saccharomyces cerevisiae
ir
3.4.11.15
Lys-Ala-4-methylcoumaryl-7-amide + H2O
-
647039
Saccharomyces cerevisiae
Lys + Ala-4-methylcoumaryl-7-amide
-
647039
Saccharomyces cerevisiae
ir
3.4.11.15
Met-7-amido-4-methylcoumarin + H2O
-
647039
Saccharomyces cerevisiae
Met + 7-amino-4-methylcoumarin
-
647039
Saccharomyces cerevisiae
ir
3.4.11.15
Met-Ala-Ser + H2O
-
647039
Saccharomyces cerevisiae
Met + Ala-Ser
-
647039
Saccharomyces cerevisiae
ir
3.4.11.15
Met-Arg-Phe + H2O
-
647039
Saccharomyces cerevisiae
Met + Arg-Phe
-
647039
Saccharomyces cerevisiae
ir
3.4.11.15
Met-Gly-Gly + H2O
-
647039
Saccharomyces cerevisiae
Met + Gly-Gly
-
647039
Saccharomyces cerevisiae
ir
3.4.11.15
additional information
generally higher activities with dipeptide-4-methylcoumaryl-7-amides than with amino-acid-4-methylcoumaryl-7-amides
647039
Saccharomyces cerevisiae
?
-
-
-
?
3.4.11.15
Pro-Phe-Arg-4-methylcoumaryl-7-amide + H2O
-
647039
Saccharomyces cerevisiae
Pro + Phe-Arg-4-methylcoumaryl-7-amide
-
647039
Saccharomyces cerevisiae
ir
3.4.11.15
Ser-7-amido-4-methylcoumarin + H2O
-
647039
Saccharomyces cerevisiae
Ser + 7-amino-4-methylcoumarin
-
647039
Saccharomyces cerevisiae
ir
3.4.11.15
Tyr-Gly-Gly-Phe-Leu + H2O
-
647039
Saccharomyces cerevisiae
Tyr + Gly-Gly-Phe-Leu
-
647039
Saccharomyces cerevisiae
ir
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.4.11.15
monomer
1 * 70000, SDS-PAGE
Saccharomyces cerevisiae