Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Kao, Y.J.; Starnes, W.L.; Behal, F.J.
    Human kidney alanine aminopeptidase: physical and kinetic properties of a sialic acid containing glycoprotein (1978), Biochemistry, 17, 2990-2994.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.11.14 0.014
-
 Met-2-naphthylamide
-
 Homo sapiens
3.4.11.14 0.04
-
 Lys-2-naphthylamide
-
 Homo sapiens
3.4.11.14 0.042
-
 Val-2-naphthylamide
-
 Homo sapiens
3.4.11.14 0.048
-
 Ile-naphthylamide
-
 Homo sapiens
3.4.11.14 0.054
-
 Arg-2-naphthylamide
-
 Homo sapiens
3.4.11.14 0.071
-
 Leu-2-naphthylamide
-
 Homo sapiens
3.4.11.14 0.123
-
 Ala-2-naphthylamide
-
 Homo sapiens
3.4.11.14 0.142
-
 Phe-2-naphthylamide
-
 Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.11.14 Co2+ 1.65fold stimulation Homo sapiens
3.4.11.14 Zinc the enzyme contains 1 mol of zinc per 113500 g of protein, the zinc is firmly bound Homo sapiens
3.4.11.14 Zinc 1 mol Zn2+ for 113500 g protein, firmly bound, not removed by chelating agents Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.11.14 119500
-
 2 * 119500, SDS-PAGE Homo sapiens
3.4.11.14 236000
-
 sedimentation equilibrium centrifugation Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.14 Homo sapiens
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.11.14 glycoprotein contains 21% carbohydrate Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.11.14
-
 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.11.14 kidney
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.11.14 76.7
-
-
 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.14 Ala-2-naphthylamide + H2O
-
 Homo sapiens Ala + 2-naphthylamine
-
 ?
3.4.11.14 Arg-2-naphthylamide + H2O
-
 Homo sapiens Arg + 2-naphthylamine
-
 ?
3.4.11.14 Ile-2-naphthylamide + H2O
-
 Homo sapiens Ile + 2-naphthylamide
-
 ?
3.4.11.14 Leu-2-naphthylamide + H2O
-
 Homo sapiens Leu + 2-naphthylamine
-
 ?
3.4.11.14 Lys-2-naphthylamide + H2O
-
 Homo sapiens Lys + 2-naphthylamine
-
 ?
3.4.11.14 Met-2-naphthylamide + H2O
-
 Homo sapiens Met + 2-naphthylamine
-
 ?
3.4.11.14 Phe-2-naphthylamide + H2O
-
 Homo sapiens Phe + 2-naphthylamine
-
 ?
3.4.11.14 Val-2-naphthylamide + H2O
-
 Homo sapiens Val + 2-naphthylamine
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.11.14 dimer 2 * 119500, SDS-PAGE Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.11.14 3.83
-
 Val-2-naphthylamide
-
 Homo sapiens
3.4.11.14 6.33
-
 Ile-2-naphthylamide
-
 Homo sapiens
3.4.11.14 11.3
-
 Lys-2-naphthylamide
-
 Homo sapiens
3.4.11.14 22.2
-
 Arg-2-naphthylamide
-
 Homo sapiens
3.4.11.14 49.5
-
 Leu-2-naphthylamide
-
 Homo sapiens
3.4.11.14 74.5
-
 Phe-2-naphthylamide
-
 Homo sapiens
3.4.11.14 95.5
-
 Met-2-naphthylamide
-
 Homo sapiens
3.4.11.14 141
-
 Ala-2-naphthylamide
-
 Homo sapiens