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Literature summary extracted from

  • Garner, C.W.; Behal, F.J.
    Human liver aminopeptidase. Role of metal ions in mechanism of action (1974), Biochemistry, 13, 3227-3233.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.4.11.14 when the enzyme is treated at 60°C with EDTA the inhibition is no longer reversible and there is a concomitant loss of zinc Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.11.14 1,10-phenanthroline 99% inhibition at 0.5 mM; Ki 0.028 mM Homo sapiens
3.4.11.14 2,2'-bipyridine 5.0 mM, 29% inhibition Homo sapiens
3.4.11.14 2,9-Dimethyl-1,10-phenanthroline 2.0 mM, 52% inhibition Homo sapiens
3.4.11.14 8-hydroxyquinoline 2.0 mM, 84% inhibition Homo sapiens
3.4.11.14 azide 92% inhibition at 10 mM Homo sapiens
3.4.11.14 cyanide 84% inhibition at 10 mM Homo sapiens
3.4.11.14 EDTA 98% inhibition at 0.001 mM Homo sapiens
3.4.11.14 EGTA 96% inhibition at 1 mM; Ki 0.012 mM Homo sapiens
3.4.11.14 KCN 10 mM, 84% inhibition Homo sapiens
3.4.11.14 Na2S 10 mM, 92% inhibition Homo sapiens
3.4.11.14 oxalate 50 mM ammonium oxalate, 71% inhibition Homo sapiens
3.4.11.14 Sulfide
-
 Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.11.14 Co2+ Km: 0.05 mM Homo sapiens
3.4.11.14 Co2+ 2.4fold activation, Co2+ binds loosely in a noncompetitive manner Homo sapiens
3.4.11.14 Zinc 8.3 nanoatoms of tightly bound zinc per mg of protein Homo sapiens
3.4.11.14 Zinc zinc is located near the substrate binding site Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.14 Homo sapiens
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.11.14 liver
-
 Homo sapiens
-

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.4.11.14 0.012
-
 EGTA
-
 Homo sapiens
3.4.11.14 0.028
-
 1,10-phenanthroline
-
 Homo sapiens
3.4.11.14 0.076
-
 Sulfide
-
 Homo sapiens