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Literature summary extracted from
- Isolation and characterization of an alanyl aminopeptidase from rat liver cytosol as a puromycin-sensitive enkephalin-degrading aminopeptidase (1998), Biol. Chem., 379, 711-719.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.11.14 | 2-mercaptoethanol | 5 mM, 2fold stimulation | Rattus norvegicus |  |
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 3.4.11.14 | inactivated after only one cycle of freezing and thawing | Rattus norvegicus |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.11.14 | 1,10-phenanthroline | 1 mM, complete inhibition | Rattus norvegicus | |
| 3.4.11.14 | actinonin | 0.1 mM, complete inactivation | Rattus norvegicus | |
| 3.4.11.14 | amastatin | 0.1 mM, complete inactivation | Rattus norvegicus | |
| 3.4.11.14 | antipain | 0.1 mM, 39% inhibition | Rattus norvegicus | |
| 3.4.11.14 | arphamenine A | 0.1 mM, 80% inactivation | Rattus norvegicus | |
| 3.4.11.14 | benzamidine | 1 mM, 14% inhibition | Rattus norvegicus | |
| 3.4.11.14 | bestatin | 0.1 mM complete inactivation | Rattus norvegicus | |
| 3.4.11.14 | Cd2+ | 1 mM, complete inhibition | Rattus norvegicus | |
| 3.4.11.14 | chymostatin | 0.1 mM, 83% inhibition | Rattus norvegicus | |
| 3.4.11.14 | Co2+ | 1 mM, complete inhibition | Rattus norvegicus | |
| 3.4.11.14 | Cu2+ | 1 mM, complete inhibition | Rattus norvegicus | |
| 3.4.11.14 | EDTA | 2 mM, 26% inhibition | Rattus norvegicus | |
| 3.4.11.14 | iodoacetamide | 1 mM, 57% inhibition | Rattus norvegicus | |
| 3.4.11.14 | iodoacetic acid | 1 mM, 11% inhibition | Rattus norvegicus | |
| 3.4.11.14 | leuhistin | 0.1 mM, complete inactivation | Rattus norvegicus | |
| 3.4.11.14 | leupeptin | 0.1 mM, 19% inhibition | Rattus norvegicus | |
| 3.4.11.14 | Mg2+ | 1 mM, 20% inhibition | Rattus norvegicus | |
| 3.4.11.14 | Mn2+ | 1 mM, 69% inhibition | Rattus norvegicus | |
| 3.4.11.14 | NEM | 1 mM, 43% inhibition | Rattus norvegicus | |
| 3.4.11.14 | Ni2+ | 1 mM, 90% inhibition | Rattus norvegicus | |
| 3.4.11.14 | phosphoramidon | 0.1 mM, 17% inhibition | Rattus norvegicus | |
| 3.4.11.14 | PMSF | 1 mM, 40% inhibition; 1 mM, 64% inhibition | Rattus norvegicus | |
| 3.4.11.14 | puromycin | 0.1 mM, complete inactivation, IC50: 0.0006 mM | Rattus norvegicus | |
| 3.4.11.14 | Zn2+ | 1 mM, complete inhibition | Rattus norvegicus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.11.14 | 0.02 | - |
Pro-Phe-Arg-7-amido-4-methylcoumarin | pH 7.5, 37°C | Rattus norvegicus | |
| 3.4.11.14 | 0.03 | - |
Arg-Arg-7-amido-4-methylcoumarin | pH 7.5, 37°C | Rattus norvegicus | |
| 3.4.11.14 | 0.11 | - |
Lys-Ala-7-amido-4-methylcoumarin | pH 7.5, 37°C | Rattus norvegicus | |
| 3.4.11.14 | 0.115 | - |
Met-7-amido-4-methylcoumarin | pH 7.5, 37°C | Rattus norvegicus | |
| 3.4.11.14 | 0.115 | - |
Lys-7-amido-4-methylcoumarin | pH 7.5, 37°C | Rattus norvegicus | |
| 3.4.11.14 | 0.17 | - |
Tyr-7-amido-4-methylcoumarin | pH 7.5, 37°C | Rattus norvegicus | |
| 3.4.11.14 | 0.22 | - |
Leu-7-amido-4-methylcoumarin | pH 7.5, 37°C | Rattus norvegicus | |
| 3.4.11.14 | 0.235 | - |
Ala-7-amido-4-methylcoumarin | pH 7.5, 37°C | Rattus norvegicus | |
| 3.4.11.14 | 0.26 | - |
Phe-7-amido-4-methylcoumarin | pH 7.5, 37°C | Rattus norvegicus | |
| 3.4.11.14 | 0.5 | - |
Pro-7-amido-4-methylcoumarin | pH 7.5, 37°C | Rattus norvegicus | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.4.11.14 | cytosol | - |
Rattus norvegicus | 5829 | - |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.11.14 | 90000 | - |
1 * 90000, SDS-PAGE in presence of 2-mercaptoethanol | Rattus norvegicus |
| 3.4.11.14 | 100000 | - |
gel filtration | Rattus norvegicus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.11.14 | Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.11.14 | - |
Rattus norvegicus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.4.11.14 | liver | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.4.11.14 | additional information | - |
- |
Rattus norvegicus |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.4.11.14 | 4°C, pH 7.5, 20 mM Tris-buffered saline, stable for several weeks | Rattus norvegicus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.11.14 | Ala-7-amido-4-methylcoumarin | - |
Rattus norvegicus | Ala + 7-amino-4-methylcoumarin | - |
? | |
| 3.4.11.14 | Arg-7-amido-4-methylcoumarin | 36% of the activity with Ala-7-amido-4-methylcoumarin | Rattus norvegicus | Arg + 7-amino-4-methylcoumarin | - |
? | |
| 3.4.11.14 | Arg-Arg-7-amido-4-methylcoumarin | 13% of the activity with Ala-7-amido-4-methylcoumarin | Rattus norvegicus | ? | - |
? | |
| 3.4.11.14 | Leu-7-amido-4-methylcoumarin | 90% of the activity with Ala-7-amido-4-methylcoumarin | Rattus norvegicus | Leu + 7-amino-4-methylcoumarin | - |
? | |
| 3.4.11.14 | Lys-7-amido-4-methylcoumarin | 93% of the activity with Ala-7-amido-4-methylcoumarin | Rattus norvegicus | Lys + 7-amino-4-methylcoumarin | - |
? | |
| 3.4.11.14 | Lys-Ala-7-amido-4-methylcoumarin | 79% of the activity with Ala-7-amido-4-methylcoumarin | Rattus norvegicus | ? | - |
? | |
| 3.4.11.14 | Met-7-amido-4-methylcoumarin | 97% of the activity with Ala-7-amido-4-methylcoumarin | Rattus norvegicus | Met + 7-amino-4-methylcoumarin | - |
? | |
| 3.4.11.14 | additional information | no activity with: Gly-Pro-7-amido-4-methylcoumarin, butyloxycarbonyl-Phe-Ser-Arg-7-amido-4-methylcoumarin, butyloxycarbonyl-Gln-Ala-Arg-7-amido-4-methylcoumarin, butyloxycarbonyl-ValPro-Arg-7-amido-4-methylcoumarin,succinyl-Ala-Ala-Ala-7-amido-4-methylcoumarin, succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin, succinyl-Gly-Pro-7-amido-4-methylcoumarin, succinyl-Gly-Pro-Leu-Gly-Pro-7-amido-4-methylcoumarin, succinyl-Ile-Ile-Trp-7-amido-4-methylcoumarin, benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin, acetyl-Asp-Glu-Val-Asp-7-amido-4-methylcoumarin | Rattus norvegicus | ? | - |
? | |
| 3.4.11.14 | Phe-7-amido-4-methylcoumarin | 84% of the activity with Ala-7-amido-4-methylcoumarin | Rattus norvegicus | Phe + 7-amino-4-methylcoumarin | - |
? | |
| 3.4.11.14 | Pro-7-amido-4-methylcoumarin | 39% of the activity with Ala-7-amido-4-methylcoumarin | Rattus norvegicus | Pro + 7-amino-4-methylcoumarin | - |
? | |
| 3.4.11.14 | Pro-Phe-Arg-7-amido-4-methylcoumarin | 20% of the activity with Ala-7-amido-4-methylcoumarin | Rattus norvegicus | ? | - |
? | |
| 3.4.11.14 | Tyr-7-amido-4-methylcoumarin + H2O | 99% of the activity with Ala-7-amido-4-methylcoumarin | Rattus norvegicus | Tyr + 7-amino-4-methylcoumarin | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.4.11.14 | monomer | 1 * 90000, SDS-PAGE in presence of 2-mercaptoethanol | Rattus norvegicus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.11.14 | 40 | - |
30 min, stable up to | Rattus norvegicus |
| 3.4.11.14 | 50 | - |
30 min, 50% loss of activity | Rattus norvegicus |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.11.14 | 0.11 | - |
Arg-Arg-7-amido-4-methylcoumarin | pH 7.5, 37°C | Rattus norvegicus | |
| 3.4.11.14 | 0.13 | - |
Arg-7-amido-4-methylcoumarin | pH 7.5, 37°C | Rattus norvegicus | |
| 3.4.11.14 | 0.97 | - |
Pro-Phe-Arg-7-amido-4-methylcoumarin | pH 7.5, 37°C | Rattus norvegicus | |
| 3.4.11.14 | 1.5 | - |
Pro-7-amido-4-methylcoumarin | pH 7.5, 37°C | Rattus norvegicus | |
| 3.4.11.14 | 4.5 | - |
Lys-Ala-7-amido-4-methylcoumarin | pH 7.5, 37°C | Rattus norvegicus | |
| 3.4.11.14 | 7.7 | - |
Tyr-7-amido-4-methylcoumarin | pH 7.5, 37°C | Rattus norvegicus | |
| 3.4.11.14 | 11.3 | - |
Leu-7-amido-4-methylcoumarin | pH 7.5, 37°C | Rattus norvegicus | |
| 3.4.11.14 | 12.8 | - |
Phe-7-amido-4-methylcoumarin | pH 7.5, 37°C | Rattus norvegicus | |
| 3.4.11.14 | 13.1 | - |
Ala-7-amido-4-methylcoumarin | pH 7.5, 37°C | Rattus norvegicus | |
| 3.4.11.14 | 14.3 | - |
Met-7-amido-4-methylcoumarin | pH 7.5, 37°C | Rattus norvegicus | |
| 3.4.11.14 | 19.5 | - |
Lys-7-amido-4-methylcoumarin | pH 7.5, 37°C | Rattus norvegicus | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.11.14 | 7.5 | - |
- |
Rattus norvegicus |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.11.14 | 5.5 | 8.5 | pH 5.5: about 55% of maximal activity, pH 8.5: about 50% of maximal activity, hydrolysis of Ala-7-amido-4-methylcoumarin | Rattus norvegicus |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.11.14 | 4 | 9.5 | 4°C, 24 h, stable | Rattus norvegicus |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 3.4.11.14 | Rattus norvegicus | chromatofocusing | 4.7 | 4.6 |
IC50 Value
| EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|---|
| 3.4.11.14 | 0.0006 | - |
0.1 mM, complete inactivation, IC50: 0.0006 mM | Rattus norvegicus | puromycin | |
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