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Literature summary extracted from

  • Rudberg, P.C.; Tholander, F.; Thunnissen, M.M.; Haeggstrom, J.Z.
    Leukotriene A4 hydrolase/aminopeptidase. Glutamate 271 is a catalytic residue with specific roles in two distinct enzyme mechanisms (2002), J. Biol. Chem., 277, 1398-1404.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.3.2.6 medicine the enzyme catalyses the hydrolysis of leukotriene A4 into the proinflammatory substance leukotriene B4 Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.3.2.6 wild-type enzyme and mutant E271Q Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.3.2.6 E271A complete loss of the epoxide hydrolase and aminopeptidase activities Homo sapiens
3.3.2.6 E271D complete loss of the epoxide hydrolase and aminopeptidase activities Homo sapiens
3.3.2.6 E271Q complete loss of the epoxide hydrolase and aminopeptidase activities Homo sapiens
3.3.2.6 G268A epoxide hydrolase activity: 97% of Km of wild type enzyme, aminopeptidase activity: 73% of Km of wild type enzyme Homo sapiens
3.3.2.6 G269A epoxide hydrolase activity: 202% of Km of wild type enzyme, aminopeptidase activity: 66% of Km of wild type enzyme Homo sapiens
3.3.2.6 M270L epoxide hydrolase activity: 21% of Km of wild type enzyme, aminopeptidase activity: 189% of Km of wild type enzyme Homo sapiens
3.3.2.6 N272A epoxide hydrolase activity: 30% of Km of wild type enzyme, aminopeptidase activity: 221% of Km of wild type enzyme Homo sapiens
3.3.2.6 Q136A epoxide hydrolase activity: 67% of Km of wild type enzyme, aminopeptidase activity: 108% of Km of wild type enzyme Homo sapiens
3.3.2.6 Q136L epoxide hydrolase activity: 179% of Km of wild type enzyme Homo sapiens
3.3.2.6 Q136N epoxide hydrolase activity: 18% of Km of wild type enzyme, aminopeptidase activity: 24% of Km of wild type enzyme Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.3.2.6 0.006 0.028 leukotriene A4 pH 8.0, wild-type enzyme in comparison to mutant enzymes Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.3.2.6 leukotriene A4 + H2O Homo sapiens
-
 leukotriene B4
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.3.2.6 Homo sapiens
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
3.3.2.6 leukotriene A4 + H2O = leukotriene B4 mechanistic models, Glu271 is specifically required for the epoxid hydrolase activity and peptidase activity Homo sapiens
a
3.3.2.6 leukotriene A4 + H2O = leukotriene B4 bifunctional enzyme acting as an epoxide hydrolase and also as an aminopeptidase Homo sapiens
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.3.2.6 leukotriene A4 + H2O
-
 Homo sapiens leukotriene B4
-
 ?
3.3.2.6 additional information
-
 Homo sapiens ?
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.3.2.6 0.21 0.47 leukotriene A4 pH 8.0, wild-type enzyme in comparison to mutant enzymes Homo sapiens