EC Number | Application | Comment | Organism |
---|---|---|---|
3.3.2.6 | medicine | the enzyme catalyses the hydrolysis of leukotriene A4 into the proinflammatory substance leukotriene B4 | Homo sapiens |
EC Number | Cloned (Comment) | Organism |
---|---|---|
3.3.2.6 | wild-type and mutant enzymes expressed in Escherichia coli | Homo sapiens |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.3.2.6 | S379A | 26% hydrolase activity, 4% peptidase activity, in comparison to 100% hydrolase- and peptidase activity of wild-type enzyme | Homo sapiens |
3.3.2.6 | S380A | 54% hydrolase activity, 68% peptidase activity, in comparison to 100% hydrolase- and peptidase activity of wild-type enzyme | Homo sapiens |
3.3.2.6 | Y378F | 173% hydrolase activity, 43% peptidase activity, in comparison to 100% hydrolase- and peptidase activity of wild-type enzyme, mutant enzyme is resistant to suicide inactivation | Homo sapiens |
3.3.2.6 | Y378Q | 34% hydrolase activity, 1.6% peptidase activity, in comparison to 100% hydrolase- and peptidase activity of wild-type enzyme, mutant enzyme is resistant to suicide inactivation | Homo sapiens |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.3.2.6 | leukotriene A4 | suicide inactivation via an apparently mechanism-based irreversible binding of leukotriene A4 to the protein in a 1:1 stoichiometry, comparison of inactivation of wild-type and mutant enzymes, Tyr378 is a residue, which is involved in the binding of leukotriene A4 | Homo sapiens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.3.2.6 | 0.006 | - |
leukotriene A4 | pH 8.0, 22°C, wild-type enzyme | Homo sapiens | |
3.3.2.6 | 0.023 | - |
leukotriene A4 | pH 8.0, 22°C, mutant Y378F | Homo sapiens |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.3.2.6 | Zinc | zinc protein | Homo sapiens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.3.2.6 | leukotriene A4 + H2O | Homo sapiens | biosynthesis of leukotriene B4 | leukotriene B4 | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.3.2.6 | Homo sapiens | - |
- |
- |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.3.2.6 | 0.692 | - |
in comparison with mutant enzymes | Homo sapiens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.3.2.6 | leukotriene A4 + H2O | - |
Homo sapiens | leukotriene B4 | - |
? | |
3.3.2.6 | leukotriene A4 + H2O | biosynthesis of leukotriene B4 | Homo sapiens | leukotriene B4 | - |
? |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.3.2.6 | 0.85 | - |
leukotriene A4 | pH 8.0, 22°C, wild-type enzyme | Homo sapiens | |
3.3.2.6 | 2.09 | - |
leukotriene A4 | pH 8.0, 22°C, mutant Y378F | Homo sapiens |