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Literature summary extracted from

  • Mueller, M.J.; Samuelsson, B.; Haeggstrom, J.Z.
    Chemical modification of leukotriene A4 hydrolase. Indications for essential tyrosyl and arginyl residues at the active site (1995), Biochemistry, 34, 3536-3543.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.3.2.6 medicine the enzyme catalyses the hydrolysis of leukotriene A4 into the proinflammatory substance leukotriene B4 Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.3.2.6 1-chloro-2,4-dinitrobenzene weak Homo sapiens
3.3.2.6 2,3-Butanedione preincubation with bestatin partially protects enzyme from inactivation Homo sapiens
3.3.2.6 Acetic anhydride complete loss of peptidase activity and epoxide hydrolase activity Homo sapiens
3.3.2.6 bestatin no influence Homo sapiens
3.3.2.6 citraconic anhydride complete loss of peptidase activity and epoxide hydrolase activity Homo sapiens
3.3.2.6 diamide/glutathione combination complete loss of peptidase activity and epoxide hydrolase activity Homo sapiens
3.3.2.6 iodoacetamide weak Homo sapiens
3.3.2.6 iodoacetic acid weak Homo sapiens
3.3.2.6 methyl methanethiosulfonate inhibition of epoxide hydrolase activity, stimulation of peptidase activity Homo sapiens
3.3.2.6 additional information not diethyl dicarbonate Homo sapiens
3.3.2.6 N-Acetylimidazole 75 min, loss of 92% peptidase activity and 78% of epoxide hydrolase activity, both activities are restored by hydroxylamine and bestatin protects Homo sapiens
3.3.2.6 N-ethylmaleimide weak Homo sapiens
3.3.2.6 Phenylglyoxal preincubation with captopril protects enzyme from inactivation Homo sapiens
3.3.2.6 Tetranitromethane preincubation with bestatin prevents the inactivation Homo sapiens
3.3.2.6 Trinitrobenzenesulfonate complete loss of peptidase activity and epoxide hydrolase activity Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.3.2.6 0.47
-
 alanine-4-nitroanilide pH 7.5, 25°C Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.3.2.6 Zinc zinc protein Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.3.2.6 leukotriene A4 + H2O Homo sapiens biosynthesis of leukotriene B4 leukotriene B4
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.3.2.6 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.3.2.6 recombinant enzyme Homo sapiens

Reaction

EC Number Reaction Comment Organism Reaction ID
3.3.2.6 leukotriene A4 + H2O = leukotriene B4 bifunctional enzyme acting as an epoxide hydrolase and also as an aminopeptidase Homo sapiens
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.3.2.6 leukotriene A4 + H2O
-
 Homo sapiens leukotriene B4
-
 ?
3.3.2.6 leukotriene A4 + H2O biosynthesis of leukotriene B4 Homo sapiens leukotriene B4
-
 ?
3.3.2.6 additional information
-
 Homo sapiens ?
-
 ?