Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Minami, M.; Bito, H.; Ohishi, N.; Tsuge, H.; Miyano, M.; Mori, M.; Wada, H.; Mutoh, H.; Shimada, S.; Izumi, T.; Abe, K.; Shimizu, T.
    Leukotriene A4 hydrolase, a bifunctional enzyme. Distinction of leukotriene A4 hydrolase and aminopeptidase activities by site-directed mutagenesis at Glu-297 (1992), FEBS Lett., 309, 353-357.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.3.2.6 medicine the enzyme catalyses the hydrolysis of leukotriene A4 into the proinflammatory substance leukotriene B4 Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.3.2.6 expression in Escherichia coli Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.3.2.6 E297A 30% hydrolase activity, 25% peptidase activity, in comparison to 100% hydrolase- and peptidase activity of wild-type enzyme Homo sapiens
3.3.2.6 E297D 17% hydrolase activity, 38% peptidase activity, in comparison to 100% hydrolase- and peptidase activity of wild-type enzyme Homo sapiens
3.3.2.6 E297K 13% hydrolase activity, 16% peptidase activity, in comparison to 100% hydrolase- and peptidase activity of wild-type enzyme Homo sapiens
3.3.2.6 E297Q 104% hydrolase activity, 14% peptidase activity, in comparison to 100% hydrolase- and peptidase activity of wild-type enzyme Homo sapiens
3.3.2.6 E319K 0% hydrolase activity, 12% peptidase activity, in comparison to 100% hydrolase- and peptidase activity of wild-type enzyme Homo sapiens
3.3.2.6 Y384F 0% hydrolase activity, 13% peptidase activity, in comparison to 100% hydrolase- and peptidase activity of wild-type enzyme Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.3.2.6 Zinc zinc protein Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.3.2.6 leukotriene A4 + H2O Homo sapiens biosynthesis of leukotriene B4 leukotriene B4
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.3.2.6 Homo sapiens
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.3.2.6 leukotriene A4 + H2O
-
 Homo sapiens leukotriene B4
-
 ?
3.3.2.6 leukotriene A4 + H2O biosynthesis of leukotriene B4 Homo sapiens leukotriene B4
-
 ?
3.3.2.6 additional information either glutamic or glutamine moiety at amino acid 297 is required for full LTA4 hydrolase activity, free carboxylic acid of glutamic acid is essential for aminopeptidase Homo sapiens ?
-
 ?