EC Number | General Stability | Organism |
---|---|---|
3.2.2.24 | Mn2+ renders the enzyme more susceptible to inactivation by dithionite in air, not Ca2+, Fe2+ or Mg2+, ADPribose protects, not ADP | Rhodospirillum rubrum |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.2.24 | ADPribose | - |
Rhodospirillum rubrum | |
3.2.2.24 | Ca2+ | - |
Rhodospirillum rubrum | |
3.2.2.24 | Dithionite | in air, inactivation | Rhodospirillum rubrum | |
3.2.2.24 | H2O2 | inactivation, to the same extent as dithionite in air | Rhodospirillum rubrum | |
3.2.2.24 | additional information | no inhibition by ADP | Rhodospirillum rubrum |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.2.2.24 | additional information | no covalently bound inorganic cofactors, inductively coupled plasma emission analysis, detection limit for Mn2+: 0.00005 mM | Rhodospirillum rubrum |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.2.24 | ADP-D-ribosyl-[dinitrogen reductase] component of nitrogenase complex | Rhodospirillum rubrum | - |
ADPribose + [dinitrogen reductase] component of nitrogenase complex | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.2.24 | Rhodospirillum rubrum | - |
- |
- |
EC Number | Oxidation Stability | Organism |
---|---|---|
3.2.2.24 | attempts to purify the enzyme using aerobic buffer are partly successful, aerobic DEAE-cellulose chromatography results in rapid loss of activity, 30 min stable to exposure to air in buffer containing DTT in the absence of dithionite, in its presence decrease of activity with time, bovine serum albumin does not protect | Rhodospirillum rubrum |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.2.24 | - |
Rhodospirillum rubrum |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.2.2.24 | 40.1 | - |
strain UR1, anaerobic preparation, prepared in the presence of 1 mM dithionite, pH 7.5 | Rhodospirillum rubrum |
3.2.2.24 | 56.6 | - |
strain UR276, anaerobic preparation, prepared in the presence of 1 mM dithionite, pH 7.5 | Rhodospirillum rubrum |
3.2.2.24 | 138 | - |
strain UR276, anaerobic preparation, pH 7.5 | Rhodospirillum rubrum |
EC Number | Storage Stability | Organism |
---|---|---|
3.2.2.24 | 4°C, purified preparation exposed to air in the absence of dithionite, 22 h | Rhodospirillum rubrum |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.2.24 | ADP-D-ribosyl-[dinitrogen reductase] component of nitrogenase complex | - |
Rhodospirillum rubrum | ADPribose + [dinitrogen reductase] component of nitrogenase complex | - |
? |