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Literature summary extracted from

  • Asagoshi, K.; Yamada, T.; Terato, H.; Ohyama, Y.; Monden, Y.; Arai, T.; Nishimura, S.; Aburatani, H.; Lindahl, T.; Ide, H.
    Distinct repair activities of human 7,8-dihydro-8-oxoguanine DNA glycosylase and formamidopyrimidine DNA glycosylase for formamidopyrimidine and 7,8-dihydro-8-oxoguanine (2000), J. Biol. Chem., 275, 4956-4964.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.2.23 0.000013
-
 DNA containing 8-hydroxyguanine residues pH 7.5, 37°C Escherichia coli
3.2.2.23 0.000038
-
 DNA containing ring-opened N7-methylguanine residues pH 7.5, 37°C Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.2.23 Zn2+ contains a single zinc finger motif near the C-terminus Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.2.23 30000
-
 x * 30000, SDS-PAGE Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.2.23 DNA containing 2,6-diamino-4-hydroxyformamidopyrimidine residues + H2O Escherichia coli repair of the major DNA lesions 7,8-dihydro-8-oxoguanine and 2,6-diamino-4-hydroxyformamidopyrimidine formed by reactive oxidative species 2,6-diamino-4-hydroxyformamidopyrimidine + DNA
-
 ?
3.2.2.23 DNA containing 8-hydroxyguanine residues + H2O Escherichia coli repair of the major DNA lesions 7,8-dihydro-8-oxoguanine and 2,6-diamino-4-hydroxyformamidopyrimidine formed by reactive oxidative species 8-hydroxyguanine + DNA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.2.23 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.2.23
-
 Escherichia coli

Reaction

EC Number Reaction Comment Organism Reaction ID
3.2.2.23 DNA containing ring-opened N7-methylguanine + H2O = deguanylated DNA + 2,6-diamino-4-hydroxy-5-(N-methyl)formamidopyrimidine reaction mechanism Escherichia coli
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.2.23 DNA containing 2,6-diamino-4-hydroxyformamidopyrimidine residues + H2O repair of the major DNA lesions 7,8-dihydro-8-oxoguanine and 2,6-diamino-4-hydroxyformamidopyrimidine formed by reactive oxidative species Escherichia coli 2,6-diamino-4-hydroxyformamidopyrimidine + DNA
-
 ?
3.2.2.23 DNA containing 8-hydroxyguanine residues + H2O comparison of repair activities of human OGG1 and Escherichia coli Fpg, enzymes show distinct preferences for the base opposite 8-oxoguanine, mechanism via Schiff base intermediate Escherichia coli 8-hydroxyguanine + DNA treatment of 7,8-dihydro-8-oxoguanine with Fpg results in delta-elimination products ?
3.2.2.23 DNA containing 8-hydroxyguanine residues + H2O readily incises dublexes with cytosine, thymine or guanine opposite, but much slower with adenine opposite 7,8-dihydro-8-oxoguanine, 2 activities: DNA-glycosylase and DNA nicking at abasic sites Escherichia coli 8-hydroxyguanine + DNA treatment of 7,8-dihydro-8-oxoguanine with Fpg results in delta-elimination products ?
3.2.2.23 DNA containing 8-hydroxyguanine residues + H2O 7,8-dihydro-8-oxoguanine-DNA Escherichia coli 8-hydroxyguanine + DNA treatment of 7,8-dihydro-8-oxoguanine with Fpg results in delta-elimination products ?
3.2.2.23 DNA containing 8-hydroxyguanine residues + H2O repair of the major DNA lesions 7,8-dihydro-8-oxoguanine and 2,6-diamino-4-hydroxyformamidopyrimidine formed by reactive oxidative species Escherichia coli 8-hydroxyguanine + DNA
-
 ?
3.2.2.23 DNA containing ring-opened N7-methylguanine residues + H2O comparison of repair activities of human OGG1 and Escherichia coli Fpg, almost no paired base-dependent repair, effect of sequence context on repair efficiency, mechanism via Schiff base intermediate Escherichia coli 2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine + DNA treatment of me-Fapy with Fpg results in delta-elimination products ?
3.2.2.23 additional information human OGG1 and Escherichia coli Fpg are structurally unrelated enzymes with different catalytic residues, OGG1: Lys-249 and Asp-268 in the hairpin-helix-hairpin-GDP motif are involved in the glycosylase/AP lyase activity, Fpg: uses a proline residue in the N-terminal region for catalysis Escherichia coli ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.2.23 ? x * 30000, SDS-PAGE Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.2.23 37
-
 assay at Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.2.23 0.03
-
 DNA containing 8-hydroxyguanine residues pH 7.5, 37°C Escherichia coli
3.2.2.23 0.085
-
 DNA containing ring-opened N7-methylguanine residues pH 7.5, 37°C Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.2.23 7.5
-
 assay at Escherichia coli