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Literature summary extracted from

  • Sidorkina, O.M.; Laval, J.
    Role of lysine-57 in the catalytic activities of Escherichia coli formamidopyrimidine-DNA glycosylase (Fpg protein) (1998), Nucleic Acids Res., 26, 5351-5357.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
3.2.2.23 K155A mutant with reduced 8-oxoguanine-DNA but unchanged Fapy-DNA glycosylase activity Escherichia coli
3.2.2.23 K57G mutant has dramatically reduced 7,8-dihydro-8-oxoguanine-DNA glycosylase activity and is poorly effective in formation of Schiff base complex with 8-oxoG/C, little effect on 2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine-DNA glycosylase activity, no effect on DNA nicking activity at abasic sites Escherichia coli
3.2.2.23 K57R slight effect of mutation on 7,8-dihydro-8-oxoguanine-DNA glycosylase activity, no effect on 2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine-DNA glycosylase activity and on DNA nicking activity at abasic sites Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.2.23 0.000004
-
 DNA containing 8-hydroxyguanine residues pH 7.8, 37°C, 34-oligomer, wild-type and K57R mutant Fpg Escherichia coli
3.2.2.23 0.000008
-
 DNA containing 8-hydroxyguanine residues pH 7.8, 37°C, 34-oligomer, K57G mutant Fpg Escherichia coli
3.2.2.23 0.000009 0.000011 DNA containing ring-opened N7-methylguanine residues pH 7.8, 37°C, wild-type, K57G and K57R mutant Fpg Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.2.23 Zn2+ possesses a zinc finger Escherichia coli
3.2.2.23 Zn2+ contains one zinc per enzyme molecule Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.2.23 31000
-
 x * 31000, SDS-PAGE Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.2.23 DNA + H2O Escherichia coli removes oxidized purines from oxidatively damaged DNA ?
-
 ?
3.2.2.23 DNA + H2O Escherichia coli BH20 removes oxidized purines from oxidatively damaged DNA ?
-
 ?
3.2.2.23 DNA containing 8-hydroxyguanine residues + H2O Escherichia coli dublex, primary physiological substrate is 7,8-dihydro-8-oxoguanine-DNA, DNA repair 8-hydroxyguanine + DNA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.2.23 Escherichia coli
-
 containing pFPG220
-
3.2.2.23 Escherichia coli BH20
-
 containing pFPG220
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.2.23 wild-type and K57G, K57R mutant Fpg Escherichia coli

Reaction

EC Number Reaction Comment Organism Reaction ID
3.2.2.23 DNA containing ring-opened N7-methylguanine + H2O = deguanylated DNA + 2,6-diamino-4-hydroxy-5-(N-methyl)formamidopyrimidine mechanism Escherichia coli
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.2.23 DNA + H2O removes oxidized purines from oxidatively damaged DNA Escherichia coli ?
-
 ?
3.2.2.23 DNA + H2O enzyme has N-glycosylase and apurinic/apyrimidinic lyase activity Escherichia coli ?
-
 ?
3.2.2.23 DNA + H2O removes oxidized purines from oxidatively damaged DNA Escherichia coli BH20 ?
-
 ?
3.2.2.23 DNA + H2O enzyme has N-glycosylase and apurinic/apyrimidinic lyase activity Escherichia coli BH20 ?
-
 ?
3.2.2.23 DNA containing 8-hydroxyguanine residues + H2O DNA glycosylase/AP lyase activity Escherichia coli 8-hydroxyguanine + DNA
-
 ?
3.2.2.23 DNA containing 8-hydroxyguanine residues + H2O 34mer oligonucleotide containing a single 7,8-dihydro-8-oxoguanine residue Escherichia coli 8-hydroxyguanine + DNA
-
 ?
3.2.2.23 DNA containing 8-hydroxyguanine residues + H2O 7,8-dihydro-8-oxoguanine opposite C dublex DNA, formation of a Schiff base intermediate, important role for Lys-57 in the 7,8-dihydro-8-oxoG-DNA glycolase activity in vitro and in vivo Escherichia coli 8-hydroxyguanine + DNA
-
 ?
3.2.2.23 DNA containing 8-hydroxyguanine residues + H2O 7,8-dihydro-8-oxoguanine-DNA Escherichia coli 8-hydroxyguanine + DNA
-
 ?
3.2.2.23 DNA containing 8-hydroxyguanine residues + H2O dublex, primary physiological substrate is 7,8-dihydro-8-oxoguanine-DNA, DNA repair Escherichia coli 8-hydroxyguanine + DNA
-
 ?
3.2.2.23 DNA containing 8-hydroxyguanine residues + H2O DNA glycosylase/AP lyase activity Escherichia coli BH20 8-hydroxyguanine + DNA
-
 ?
3.2.2.23 DNA containing 8-hydroxyguanine residues + H2O 34mer oligonucleotide containing a single 7,8-dihydro-8-oxoguanine residue Escherichia coli BH20 8-hydroxyguanine + DNA
-
 ?
3.2.2.23 DNA containing 8-hyroxyguanine + H2O
-
 Escherichia coli ?
-
 ?
3.2.2.23 DNA containing ring-opened N7-methylguanine residues + H2O
-
 Escherichia coli 2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine + DNA
-
 ?
3.2.2.23 DNA containing ring-opened N7-methylguanine residues + H2O
-
 Escherichia coli BH20 2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine + DNA
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.2.23 ? x * 31000, SDS-PAGE Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.2.23 37
-
 assay at Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.2.23 0.000167
-
 DNA containing 8-hydroxyguanine residues pH 7.8, 37°C, 34-oligomer, K57G mutant Fpg Escherichia coli
3.2.2.23 0.00167
-
 DNA containing 8-hyroxyguanine pH 7.8, 37°C, 34-oligomer, K57R mutant Fpg Escherichia coli
3.2.2.23 0.002
-
 DNA containing ring-opened N7-methylguanine residues pH 7.8, 37°C, K57G and K57R mutant Fpg Escherichia coli
3.2.2.23 0.00717
-
 DNA containing 8-hydroxyguanine residues pH 7.8, 37°C, 34-oligomer, wild-type Fpg Escherichia coli
3.2.2.23 0.00833
-
 DNA containing ring-opened N7-methylguanine residues pH 7.8, 37°C, wild-type Fpg Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.2.23 7.8
-
 assay at Escherichia coli