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Literature summary extracted from

  • Gao, M.J.; Murphy, T.M.
    Alternative forms of formamidopyrimidine-DNA glycosylase from Arabidopsis thaliana (2001), Photochem. Photobiol., 73, 128-134.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.2.23 isolation of 2 cDNA clones: alternative splicing products AtFPG-1 and -2 of a single gene with different C-terminal sequences, amino acid sequences, expression of both proteins in Escherichia coli BL21(DE3) Arabidopsis thaliana

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.2.23 additional information no zinc finger Arabidopsis thaliana

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.2.23 32400
-
 x * 32400, AtFPG-2, calculated from the amino acid sequence Arabidopsis thaliana
3.2.2.23 34000
-
 x * 34000, AtFPG-2, SDS-PAGE Arabidopsis thaliana
3.2.2.23 44800
-
 x * 44800, AtFPG-1, calculated from the amino acid sequence Arabidopsis thaliana
3.2.2.23 46000
-
 x * 46000, AtFPG-1, SDS-PAGE Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.2.23 DNA + H2O Arabidopsis thaliana catalyzes the initial steps in the repair of DNA containing oxidized purines ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.2.23 Arabidopsis thaliana O80358 AtFPG-1, enzyme exists in 2 splicing variants: AtFPG-1 and -2
-
3.2.2.23 Arabidopsis thaliana O80358 AtFPG-2, enzyme exists in 2 splicing variants: AtFPG-1 and -2
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.2.23 recombinant AtFPG-1, expressed in Escherichia coli BL21(DE3) Arabidopsis thaliana
3.2.2.23 recombinant AtFPG-2, expressed in Escherichia coli BL21(DE3) Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.2.23 DNA + H2O both AtFPG-1 and -2 cleave DNA containing apurinic sites and UV-irradiated and oxidized DNA Arabidopsis thaliana ?
-
 ?
3.2.2.23 DNA + H2O catalyzes the initial steps in the repair of DNA containing oxidized purines Arabidopsis thaliana ?
-
 ?
3.2.2.23 DNA containing 8-hydroxyguanine residues + H2O AtFPG-1, but not AtFPG-2, cleaves double-stranded oligonucleotides containing 8-oxoguanine Arabidopsis thaliana 8-hydroxyguanine + DNA
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.2.23 ? x * 32400, AtFPG-2, calculated from the amino acid sequence Arabidopsis thaliana
3.2.2.23 ? x * 44800, AtFPG-1, calculated from the amino acid sequence Arabidopsis thaliana
3.2.2.23 ? x * 46000, AtFPG-1, SDS-PAGE Arabidopsis thaliana
3.2.2.23 ? x * 34000, AtFPG-2, SDS-PAGE Arabidopsis thaliana