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Literature summary extracted from

  • Rabow, L.E.; Kow, Y.W.
    Mechanism of action of base release by Escherichia coli Fpg protein: Role of lysine 155 in catalysis (1997), Biochemistry, 36, 5084-5096.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.2.23 expression of K155A mutant and wild-type Fpg in Escherichia coli BL21(DE3) and BLR(DE3) Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
3.2.2.23 K155A mutant with 50fold decreased activity with 7-hydro-8-oxoguanine-DNA as substrate, only 3-4fold decreased activity with 7-methylformamidopyrimidine-DNA, increased AP lyase activity Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.2.23 0.0000009
-
 DNA pH 7.5, 20°C, 24-oligomer DNA containing apurinic/apyrimidinic sites, lyase activity, K155A mutant Fpg Escherichia coli
3.2.2.23 0.0000069
-
 DNA containing 7-hydro-8-oxoguanine residues pH 7.5, 37°C, 24-oligomer, wild-type Fpg Escherichia coli
3.2.2.23 0.0000075
-
 DNA containing 7-hydro-8-oxoguanine residues pH 7.5, 37°C, 24-oligomer, K155A mutant Fpg Escherichia coli
3.2.2.23 0.000019
-
 DNA pH 7.5, 20°C, 24-oligomer DNA containing AP sites, lyase activity, wild-type Fpg Escherichia coli
3.2.2.23 0.000023
-
 DNA containing ring-opened N7-methylguanine residues pH 7.5, 37°C, 23-oligomer, wild-type Fpg Escherichia coli
3.2.2.23 0.000029
-
 DNA containing ring-opened N7-methylguanine residues pH 7.5, 37°C, 23-oligomer, K155A mutant Fpg Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.2.23 35000
-
 1 * 35000, SDS-PAGE Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.2.23 DNA + H2O Escherichia coli removes oxidized purines from oxidatively damaged DNA ?
-
 ?
3.2.2.23 DNA + H2O Escherichia coli DNA base excision repair enzyme ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.2.23 Escherichia coli P50465 strain 5H11S
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.2.23 wild-type and K155A mutant Fpg, expressed in Escherichia coli BLR(DE3) Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.2.23 0.054
-
 polymeric DNA containing ring-opened N7-methylguanine residues as substrate, wild-type Fpg Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.2.23 DNA + H2O with apurinic/apyrimidinic lyase activity, catalyzes beta and delta elimination reactions Escherichia coli ?
-
 ?
3.2.2.23 DNA + H2O removes oxidized purines from oxidatively damaged DNA Escherichia coli ?
-
 ?
3.2.2.23 DNA + H2O enzyme has N-glycosylase and apurinic/apyrimidinic lyase activity Escherichia coli ?
-
 ?
3.2.2.23 DNA + H2O DNA base excision repair enzyme Escherichia coli ?
-
 ?
3.2.2.23 DNA containing 2'-deoxy-8-oxonebularine residues + H2O 23mer oligonucleotide containing a single site, very poor substrate Escherichia coli ?
-
 ?
3.2.2.23 DNA containing 2,6-diamino-4-hydroxy-5-formamidopyrimidine residues + H2O FapyGua Escherichia coli 2,6-diamino-4-hydroxy-5-formamidopyrimidine + DNA
-
 ?
3.2.2.23 DNA containing 4,6-diamino-5-formamidopyrimidine residues + H2O FapyAde Escherichia coli 4,6-diamino-5-formamidopyrimidine + DNA
-
 ?
3.2.2.23 DNA containing 7-hydro-8-oxoguanine residues + H2O 24-oligomer, Lys-155 directly interacts with the C8 oxygen of 8-oxopurines involving proton transfer or transient formation of an ion pair between enzyme and substrate, mechanism Escherichia coli DNA + 7-hydro-8-oxoguanine
-
 ?
3.2.2.23 DNA containing 7-hydro-8-oxoguanine residues + H2O DNA glycosylase/AP lyase activity Escherichia coli DNA + 7-hydro-8-oxoguanine
-
 ?
3.2.2.23 DNA containing ring-opened N7-methylguanine residues + H2O 23-oligomer Escherichia coli 2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine + DNA
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.2.23 monomer 1 * 35000, SDS-PAGE Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.2.23 37
-
 assay at Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.2.23 0.000383
-
 DNA containing 7-hydro-8-oxoguanine residues pH 7.5, 37°C, 24-oligomer, K155A mutant Fpg Escherichia coli
3.2.2.23 0.0012
-
 DNA containing ring-opened N7-methylguanine residues pH 7.5, 37°C, 23-oligomer, K155A mutant Fpg Escherichia coli
3.2.2.23 0.00383
-
 DNA containing ring-opened N7-methylguanine residues pH 7.5, 37°C, 23-oligomer, wild-type Fpg Escherichia coli
3.2.2.23 0.0185
-
 DNA containing 7-hydro-8-oxoguanine residues pH 7.5, 37°C, 24-oligomer, wild-type Fpg Escherichia coli
3.2.2.23 0.0568
-
 DNA pH 7.5, 20°C, 24-oligomer DNA containing AP sites, lyase activity, wild-type Fpg Escherichia coli
3.2.2.23 0.0883
-
 DNA pH 7.5, 20°C, 24-oligomer DNA containing AP sites, lyase activity, K155A mutant Fpg Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.2.23 7.5
-
 assay at Escherichia coli