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Literature summary extracted from
- Purification and characterization of novel ribosome inactivating proteins, alpha- and beta-pisavins, from seeds of the garden pea Pisum sativum (1998), Biochem. Biophys. Res. Commun., 253, 135-142.
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.2.22 | 18700 | - |
beta-pisavin, gel filtration | Pisum sativum |
| 3.2.2.22 | 20500 | - |
alpha-pisavin, gel filtration | Pisum sativum |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.2.22 | rRNA + H2O | Pisum sativum | - |
? | - |
? |  |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.2.22 | Pisum sativum | - |
garden pea, var. arvense Poir | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.2.22 | - |
Pisum sativum |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.2.2.22 | seed | - |
Pisum sativum | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.2.22 | additional information | - |
specific activity 10000 U/mg, 1 unit is the concentration of protein in mg/ml inhibiting protein synthesis by 50% | Pisum sativum |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.2.22 | additional information | activity toward tRNA | Pisum sativum | ? | - |
? | |
| 3.2.2.22 | rRNA + H2O | - |
Pisum sativum | ? | - |
? | |
| 3.2.2.22 | supercoiled double-stranded DNA + H2O | - |
Pisum sativum | nicked and linear forms of DNA | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.2.22 | alpha-pisavin | - |
Pisum sativum |
| 3.2.2.22 | beta-pisavin | - |
Pisum sativum |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 3.2.2.22 | Pisum sativum | isoelectric focusing, pI similar to trichosanthin significantly higher than 9.3, out of the range of pI standards | - |
9.3 |
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