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Literature summary extracted from

  • Imanaka, T.; Kuriki, T.
    Pattern of action of Bacillus stearothermophilus neopullulanase on pullulan (1989), J. Bacteriol., 171, 369-374.
    View publication on PubMedView publication on EuropePMC

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.135 pullulan + H2O Geobacillus stearothermophilus
-
panose + ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.135 Geobacillus stearothermophilus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.135 additional information enzyme hydrolyzes not only alpha-1,4-glucosidic linkages but also specific alpha-1,6-glucosidic linkages of several branched oligosaccharides Geobacillus stearothermophilus ?
-
?
3.2.1.135 pullulan + H2O
-
Geobacillus stearothermophilus panose + ?
-
?
3.2.1.135 pullulan + H2O
-
Geobacillus stearothermophilus panose + ? in the first step, the enzyme hydrolyzes only alpha-1,4-glucosidic linkages on the nonreducing side of alpha-1,6-linkages of pullulan and produces panose and several intermediate products composed of some panose units. In the second step, taking 62-O-alpha-(63-O-alpha-glucosyl-maltotriosyl)-maltose as an example of one of the intermediate products, the enzyme hydrolyzes either alpha-1,4-, or alpha-1,6-linkages and produces panose or 63-O-alpha-glucosyl-maltotriose plus maltose, respectively. In the third step, the alpha-1,4-linkage of the 63-O-alpha-glucosyl-maltotriose is hydrolyzed by the enzyme, and D-glucose and another panose are produced ?
3.2.1.135 pullulan + H2O
-
Geobacillus stearothermophilus panose + ? + maltose + D-glucose, in a molar ratio of 3:1:1, small amount ?