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Literature summary extracted from

  • Lee, H.S.; Kim, M.S.; Cho, H.S.; Kim, J.I.; Kim, T.J.; Choi, J.H.; Park, C.; Oh, B.H.; Park, K.H.
    Cyclomaltodextrinase, neopullulanase, and maltogenic amylase are nearly indistinguishable from each other (2002), J. Biol. Chem., 277, 21891-21897.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.54 expression of wild-type and mutant enzymes in Escherichia coli strain MC1061 Bacillus sp. (in: Bacteria)
3.2.1.133 cloned and overproduced in Escherichia coli MC1061 Thermus sp.

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.54 10 mg/ml purified recombinant enzyme in 10 mM HEPES, pH 7.5 and 1 mM 2-mercaptoethanol, hanging drop vapour diffusion method by addition of equal amount of 0.002 ml of precipitant solution containing 50% PEG 200, and 0.1 M HEPES, pH 7.0, equilibration against 1 ml, 22°C, X-ray diffraction structure determination and analysis at 3.3 A resolution Bacillus sp. (in: Bacteria)
3.2.1.133 mutant E357L, space group P6(1), a = b = 119.09, c = 270.20 Thermus sp.

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.54 additional information construction of a mutant enzyme lacking the C-terminal domain, expression as insoluble protein in Escherichia coli Bacillus sp. (in: Bacteria)
3.2.1.133 E357L site-directed mutagenesis Thermus sp.
3.2.1.133 W47A site-directed mutagenesis Thermus sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.54 1.43
-
 beta-cyclomaltodextrin pH 6.0, dimer Bacillus sp. (in: Bacteria)
3.2.1.54 8.68
-
 beta-cyclomaltodextrin pH 7.0, dodecamer Bacillus sp. (in: Bacteria)
3.2.1.133 additional information
-
 additional information Km 131 mg/ml, starch as substrate, mutant W47A Thermus sp.
3.2.1.133 additional information
-
 additional information Km 73.5 mg/ml, starch as substrate, wild-type Thermus sp.
3.2.1.133 0.174
-
 beta-cyclodextrin pH 6.0, 60°C, wild-type Thermus sp.
3.2.1.133 2.03
-
 beta-cyclodextrin pH 6.0, 60°C, mutant W47A Thermus sp.

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.54 Bacillus sp. (in: Bacteria)
-
 alcalophilic strain I-5
-
3.2.1.133 Thermus sp.
-
-
-
3.2.1.135 Geobacillus stearothermophilus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.54 recombinant enzyme from Escherichia coli by ammonium sulfate precipitation and Sepharose Q chromatography Bacillus sp. (in: Bacteria)
3.2.1.133 mutant E357L Thermus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.54 alpha-cyclomaltodextrin + H2O
-
 Bacillus sp. (in: Bacteria) alpha-D-glucose + maltose
-
 ?
3.2.1.54 beta-cyclomaltodextrin + H2O
-
 Bacillus sp. (in: Bacteria) alpha-D-glucose + maltose
-
 ?
3.2.1.54 gamma-cyclomaltodextrin + H2O
-
 Bacillus sp. (in: Bacteria) alpha-D-glucose + maltose
-
 ?
3.2.1.54 pullulan + H2O
-
 Bacillus sp. (in: Bacteria) ?
-
 ?
3.2.1.54 starch + H2O
-
 Bacillus sp. (in: Bacteria) alpha-D-glucose + maltose
-
 ?
3.2.1.133 cyclomaltodextrin + H2O
-
 Thermus sp. alpha-maltose + alpha-D-glucose
-
 ?
3.2.1.133 additional information nearly indistinguishable from cyclomaltodextrinase from Bacillus sp. and Bacillus stearothermophilus neopullulanase, distinguished from typicsl alpha-amylases by containing a novel N-terminal domain and exhibiting preferential substrate specificities for cyclomaltodextrins over starch Thermus sp. ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.54 dimer domain-swapped dimer Bacillus sp. (in: Bacteria)
3.2.1.54 dodecamer dodecameric assembly of 6 copies of the dimer, most active form Bacillus sp. (in: Bacteria)
3.2.1.54 More pH-dependent association and dissociation of the enzyme dimeric units, structure determination and analysis, enzyme has a beta-stranded N-terminal domain, residues 1-123, a central (alphabeta)8 barrel domain, and a beta-stranded C-terminal domain, residues 505-583 Bacillus sp. (in: Bacteria)
3.2.1.133 dimer
-
 Thermus sp.

Synonyms

EC Number Synonyms Comment Organism
3.2.1.54 CDase
-
 Bacillus sp. (in: Bacteria)
3.2.1.133 ThMA
-
 Thermus sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.54 0.7
-
 beta-cyclomaltodextrin pH 7.0, dodecamer Bacillus sp. (in: Bacteria)
3.2.1.54 1.68
-
 beta-cyclomaltodextrin pH 6.0, dimer Bacillus sp. (in: Bacteria)
3.2.1.133 126
-
 beta-cyclodextrin
-
 Thermus sp.
3.2.1.133 180
-
 beta-cyclodextrin pH 6.0, 60°C, mutantW47A Thermus sp.
3.2.1.133 249
-
 starch pH 6.0, 60°C, wild-type Thermus sp.
3.2.1.133 335
-
 starch pH 6.0, 60°C, mutant W47A Thermus sp.