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Literature summary extracted from

  • Kim, J.S.; Cha, S.S.; Kim, H.J.; Kim, T.J.; Ha, N.C.; Oh, S.T.; Cho, H.S.; Cho, M.J.; Kim, M.J.; Lee, H.S.; Kim, J.W.; Choi, K.Y.; Park, K.H.; Oh, B.H.
    Crystal structure of a maltogenic amylase provides insights into a catalytic versatility (1999), J. Biol. Chem., 274, 26279-26286.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.133 biotechnology natural design of the transglycosylation activtiy of the enzyme at high concentrations of acceptor sugar molecules may now be altered for biotechnological applications to produce branched oligosaccharides with higher efficiency Thermus sp.

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.133
-
 Thermus sp.

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.133
-
 Geobacillus stearothermophilus
3.2.1.133 crystals obtained by vapor diffusion, space group P6(1), cell dimensions of a = b = 118.04 A, c = 266.88 A Thermus sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.133 starch + H2O Geobacillus stearothermophilus
-
 alpha-maltose + ?
-
 ?
3.2.1.133 starch + H2O Thermus sp. catalyzes the hydrolysis of starch material, central role in carbohydrate metabolism alpha-maltose + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.133 Geobacillus stearothermophilus
-
-
-
3.2.1.133 Thermus sp.
-
 atomic coordinates and structure factors, PDB: 1SMA
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.133 acarbose + H2O
-
 Geobacillus stearothermophilus acarviosine-glucose + alpha-D-glucose
-
 ?
3.2.1.133 acarbose + H2O
-
 Thermus sp. acarviosine-glucose + alpha-D-glucose
-
 ?
3.2.1.133 beta-cyclodextrin + H2O
-
 Geobacillus stearothermophilus alpha-maltose + alpha-D-glucose
-
 ?
3.2.1.133 beta-cyclodextrin + H2O prefers cyclodextrins to starch or pullulan as substrate Thermus sp. alpha-maltose + alpha-D-glucose
-
 ?
3.2.1.133 additional information exhibits dual activity of alpha-D-(1,4)- and alpha-D-(1,6)- glycosidic bond cleavages, shows activity of alpha-D(1,4)- to alpha-D-(1,3), alpha-D-(1,4), or alpha-D-(1,6)-transglycosylation and cleaves acarbose, a pseudotetrasaccharide competitive inhibitor of alpha-amylases Thermus sp. ?
-
 ?
3.2.1.133 starch + H2O
-
 Geobacillus stearothermophilus alpha-maltose + ?
-
 ?
3.2.1.133 starch + H2O
-
 Thermus sp. alpha-maltose + ?
-
 ?
3.2.1.133 starch + H2O catalyzes the hydrolysis of starch material, central role in carbohydrate metabolism Thermus sp. alpha-maltose + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.133 homodimer
-
 Thermus sp.

Synonyms

EC Number Synonyms Comment Organism
3.2.1.133 BSMA
-
 Geobacillus stearothermophilus
3.2.1.133 ThMA
-
 Thermus sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.133 60
-
-
 Geobacillus stearothermophilus
3.2.1.133 60
-
-
 Thermus sp.