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Literature summary extracted from

  • Colomer-Pallas, A.; Pereira, Y.; Petit-Glatron, M.F.; Chambert, R.
    Calcium triggers the refolding of Bacillus subtilis chitosanase (2003), Biochem. J., 369, 731-738.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
3.2.1.132 heat treatment leads at a concentration of 0.0106 mM protein at 75°C to a irreversible unfolding, but this is reversible when the protein concentation is 20 times lower Bacillus subtilis
3.2.1.132 unfolding and refolding kinetics with urea are both monophasic half-life for unfolding in 6 M urea is 78.8 s and in 1.75 M urea 73.7 s Bacillus subtilis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.132 extracellular
-
 Bacillus subtilis
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.132 Ca2+ influences folding an stability of newly translocated protein, one weak Ca2+-binding site with a KA of 300 M-1 Bacillus subtilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.132 chitosan + H2O Bacillus subtilis
-
 ?
-
 ?
3.2.1.132 chitosan + H2O Bacillus subtilis GM9804
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.132 Bacillus subtilis
-
 GM9804
-
3.2.1.132 Bacillus subtilis GM9804
-
 GM9804
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.132 chitosan + H2O
-
 Bacillus subtilis ?
-
 ?
3.2.1.132 chitosan + H2O
-
 Bacillus subtilis GM9804 ?
-
 ?