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Literature summary extracted from

  • Chiang, C.L.; Chang, C.T.; Sung, H.Y.
    Purification and properties of chitosanase from a mutant of Bacillus subtilis IMR-NK1 (2003), Enzyme Microb. Technol., 32, 260-267.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.132 Hg2+
-
 Bacillus subtilis
3.2.1.132 N-bromosuccinimide
-
 Bacillus subtilis
3.2.1.132 p-hydroxymercuribenzoic acid
-
 Bacillus subtilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.132 36000
-
 gel filtration Bacillus subtilis
3.2.1.132 41000
-
 1 * 41000, SDS-PAGE Bacillus subtilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.132 chitosan + H2O Bacillus subtilis
-
 ?
-
 ?
3.2.1.132 chitosan + H2O Bacillus subtilis IMR-NK1
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.132 Bacillus subtilis
-
 IMR-NK1
-
3.2.1.132 Bacillus subtilis IMR-NK1
-
 IMR-NK1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.132
-
 Bacillus subtilis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.132 2819
-
 after DEAE-Sephacel ion exchange chromatography Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.132 chitosan + H2O
-
 Bacillus subtilis ?
-
 ?
3.2.1.132 chitosan + H2O
-
 Bacillus subtilis IMR-NK1 ?
-
 ?
3.2.1.132 chitosan + H2O degrades 60-94% deacetylated chitosan most effectively Bacillus subtilis additional information end products of chitosan hydrolysis are chitobiose, chitotetraose and some chitooligosaccharides with a longer chain length ?
3.2.1.132 chitosan + H2O degrades 60-94% deacetylated chitosan most effectively Bacillus subtilis IMR-NK1 additional information end products of chitosan hydrolysis are chitobiose, chitotetraose and some chitooligosaccharides with a longer chain length ?
3.2.1.132 colloidal chitin + H2O
-
 Bacillus subtilis ?
-
 ?
3.2.1.132 colloidal chitin + H2O
-
 Bacillus subtilis IMR-NK1 ?
-
 ?
3.2.1.132 glycol chitosan + H2O
-
 Bacillus subtilis ?
-
 ?
3.2.1.132 glycol chitosan + H2O
-
 Bacillus subtilis IMR-NK1 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.132 monomer 1 * 41000, SDS-PAGE Bacillus subtilis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.132 45
-
-
 Bacillus subtilis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.132 30
-
 rather stable for 20 min Bacillus subtilis
3.2.1.132 40
-
 rather stable for 20 min Bacillus subtilis
3.2.1.132 45
-
 gradual inactivation of the enzyme Bacillus subtilis
3.2.1.132 50
-
 gradual inactivation of the enzyme Bacillus subtilis
3.2.1.132 55
-
 significant loss in activity Bacillus subtilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.132 4
-
-
 Bacillus subtilis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.132 5 9 stable at 25°C for 1 h Bacillus subtilis